高等学校化学学报

高等学校化学学报

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毛细管电泳电致化学发光灵敏检测毒品类生物碱及在尿样分析中的应用

刘彦明, 田伟   

  1. 信阳师范学院化学化工学院, 信阳 464000
  • 收稿日期:2008-10-06 修回日期:1900-01-01 出版日期:2009-01-10 发布日期:2009-01-10

Sensitive Determination of Drug Alkaloids by Capillary Electrophoresis with Electrochemiluminescence Detection and Its Applications to Analysis of Human Urine

LIU Yan-Ming*, TIAN Wei   

  1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China

  • Received:2008-10-06 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10

PDF (PC)

被引次数

14

摘要： 本文基于盐酸甲基麻黄碱、蒂巴因、磷酸可待因和乙酰可待因等4种分析物对三联吡啶钌电致化学发光具有增强作用的特性, 同时结合离子液体的使用, 提出了一种CE-ECL同时分离检测的新方法. 对电泳分离条件、电致化学发光检测条件和离子液体的影响进行了系统优化, 将提出的新方法成功应用于人尿样中4种毒品含量的分析, 结果令人满意.

关键词: 毛细管电泳, 电致化学发光检测, 毒品类生物碱, 离子液体, 尿样分析

Abstract: A new approach for the determination of methylephedrine hydrochloride(ME), thebaine, codeine phosphate(CP), and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate, BMIMBF4). The conditions for the CE separation, ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions, the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME, thebaine, CP, and AC were 2.1×10-8, 1.4×10-7, 6.3×10-8, and 3.6×10-8 mol/L(S/N=3), respectively. The limits of quantitation(LOQ, S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME, 3.6 ×10-6 mol/L for thebaine, 6.5×10-7 mol/L for CP, and 4.6×10-7 mol/L for AC, respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.

Key words: Capillary electrophoresis, Electrochemiluminescence detection, Drug alkaloids, Ionic liquid, Urine analysis

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