纳米银比色法检测多巴胺

doi:10.7503/cjcu20150273

摘要/Abstract

摘要：

在银纳米粒子存在下, 多巴胺可还原硝酸银生成银, 导致银纳米粒子粒径增大, 从而使溶液颜色发生改变. 基于此, 提出了一种用于检测多巴胺的纳米银比色法. 随着多巴胺浓度的增大, 溶液的颜色由浅黄色逐渐变为深黄色, 银纳米粒子溶液的吸收峰发生红移且吸光度增大. 在最优实验条件下, 该方法检测多巴胺的线性范围为0.05~16 μmol/L, 检出限为0.04 μmol/L. 该方法操作简单、灵敏且选择性良好, 可用于人血清中多巴胺的检测.

关键词: 银纳米粒子, 比色法, 多巴胺

Abstract:

A simple and sensitive colorimetric method for the detection of dopamine was proposed based on the redox reaction between silver ion and dopamine, which was catalyzed by silver nanoparticles(AgNPs), causing the color change. The increase of dopamine concentration led to red shift of absorption peak and increase of the absorbance. Meanwhile, the color of the solution changed from pale yellow and deep yellow. Under optimal conditions, a linear range of 0.05—16 μmol/L between the absorbance and dopamine concentration and a detection limit of 0.04 μmol/L was obtained. The proposed method was sensitive, simple, low-cost and selective, which could be applied for the detection of dopamine in human serum.

Key words: Silver nanoparticles, Colorimetric, Dopamine

中图分类号:

O657

TrendMD:

冯娟娟, 赵祎曼, 王海燕. 纳米银比色法检测多巴胺. 高等学校化学学报, 2015, 36(7): 1269.

FENG Juanjuan, ZHAO Yiman, WANG Haiyan. Colorimetric Detection of Dopamine Based on Silver Nanoparticles^†. Chem. J. Chinese Universities, 2015, 36(7): 1269.

图/表 8

Scheme 1 Colorimetric detection of Dopamine(DA) using citrate-stabilized AgNPs

Fig.1 UV-Vis spectra of AgNPs stored for a fixed period(A) and AgNPs with some additions(B)Inset of (A): plot of the absorbance of AgNPs vs. time.

Fig.2 SEM(A, A'), TEM(B, B') images and size distributions(C, C') measured by DLS of the AgNPs in the absence(A—C) and presence(A'—C') of DA

Fig.3 Effects of amount of AgNO3(A), reaction time(B) and pH value(C) on the absorbance of AgNPs solution

Fig.4 Visual color changes of AgNPs solution in the presence of different concentrations of DA(A), calibration curve for detection of DA(B) and effect of DA concentration on the peak position(C)(A) cDA/(μmol·L-1); a. 0; b. 0.05; c. 1; d. 4; e. 7; f. 10; g. 13; h. 16; i. 19; j. 22; k. 25; l .28.Inset of (B): plots of the absorbance of AgNPs vs. DA concentrations ranging from 0.05 μmol/L to 31 μmol/L.

Table 1 Comparison of analytical features of colorimetric methods for detection of DA

NPs Probe	Linear range/(μmol·L^-1)	LOD/(μmol·L^-1)	Ref.
AuNPs	0.5—10	0.20	[6]
AHMT-AuNPs^*	0.2—1.1	0.07	[23]
Au nanorods	0.2—12	0.047	[24]
AgNPs	0—0.6	0.06	[26]
AgNPs-Ag⁺	0.05—16	0.04	This work

Fig.5 Changes in the absorbance of AgNPs with 10 μmol/L DA or other analytes(A) and UV-Vis spectra(B) of AgNPs with 10 μmol/L DA(a) and 100 μmol/L AA(b)(A) a. DA; b. Lys; c. Phe; d. Gly; e. Glu; f. UA; g. CA; h. Catechol; i. K+; j. Na+; k. Cu2+. Inset of (B): visual color changes.

Table 2 Detection of dopamine in human serum(n=5)

Sample	Added/(μmol·L^-1)	Measured/(μmol·L^-1)	Recovery(%)	RSD(%)
Serum 1	0.3	0.296	98.7	3.0
Serum 2	0.3	0.31	103.3	6.4
Serum 3	0.3	0.294	98	3.0

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