高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (3): 551.doi: 10.7503/cjcu20120687

• 分析化学 • 上一篇    下一篇

以聚硫堇为电化学探针的非标记型核酸适配体传感器

杨绍明, 查文玲, 李红, 孙清, 刘斌, 郑龙珍   

  1. 华东交通大学基础学院化学化工系, 南昌 330013
  • 收稿日期:2012-07-20 出版日期:2013-03-10 发布日期:2013-02-18
  • 通讯作者: 杨绍明,男,博士,副教授,主要从事电分析化学研究.E-mail:yangsm@yahoo.cn E-mail:yangsm@yahoo.cn
  • 基金资助:

    国家自然科学基金(批准号:21065004)、江西省自然科学基金(批准号:2009GQH0022)和江西省教育厅科技项目(批准号:GJJ12304)资助.

Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe

YANG Shao-Ming, ZHA Wen-Ling, LI Hong, SUN Qing, LIU Bin, ZHENG Long-Zhen   

  1. Department of Chemistry and Chemical Engineering, School of Basic Sciences, East China Jiaotong University, Nanchang 330013, China
  • Received:2012-07-20 Online:2013-03-10 Published:2013-02-18
  • Contact: YANG Shao-ming E-mail:yangsm@yahoo.cn

PDF (PC)

被引次数

14

摘要：

采用电聚合法制备了聚硫堇氧化还原电化学探针, 以金纳米粒子为固定核酸适配体的载体构建了非标记型核酸适配体传感器. 用电化学阻抗谱对传感器的组装过程进行了监测, 用循环伏安法和差分脉冲伏安法考察了传感器的电化学行为. 结果表明, 该传感器对凝血酶的检测在1.0 pg/mL～500 ng/mL范围内呈良好的线性关系, 相关系数为0.998, 检出限为0.38 pg/mL. 该传感器制备简单、 灵敏度高且抗干扰能力强.

关键词: 聚硫堇, 金纳米粒子, 核酸适配体, 凝血酶, 传感器

Abstract:

A lable-free aptamer biosensor was fabricated with the electropolymerized poly(thionine) as a redox electrochemical probe and gold nanoparticles as the immobilization matrix for aptamer. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of the aptamer biosensor. The electrochemical behavior of the aptamer biosensor was studied by the cyclic voltammetry(CV) and differential pulse voltammetry(DPV). The biosensor shows a good linear range of 1.0 pg/mL—500 ng/mL for thrombin, R=0.998, with a detection limit of 0.38 pg/mL. The biosensor has simple preparation procedure, high sensitivity and strong anti-interference ability.

Key words: Poly(thionine), Gold nanoparticles, Aptamer, Thrombin, Biosensor

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