高等学校化学学报

高等学校化学学报

• 研究简报 • 上一篇    下一篇

毛细管电泳免疫化学发光高灵敏检测人血清中肿瘤标志物甲胎蛋白

刘彦明1; 穆海贝1; 王成全1; 陈永红2; 李福荣3; 王军华4; 程介克4   

  1. 1. 信阳师范学院化学化工学院, 信阳 464000;
    2. 信阳市中心医院, 信阳 464000;
    3. 信阳师范学院生命科学学院, 信阳 464000;
    4. 武汉大学化学与分子科学学院, 武汉 430072
  • 收稿日期:2006-01-26 修回日期:1900-01-01 出版日期:2006-07-10 发布日期:2006-07-10
  • 通讯作者: 刘彦明

Highly Sensitive Chemiluminescence Detection of Tumor Marker alpha-Fetoprotein in Human Sera with Capillary Electrophoresis Immunoassay

LIU Yan-Ming1*; MU Hai-Bei1; WANG Cheng-Quan1; CHEN Yong-Hong2; LI Fu-Rong3; WANG Jun-Hua4; CHENG Jie-Ke4   

  1. 1. College of Chemistry and Chemical Engineering, Xinyang Normal University,
    Xinyang 464000, China;
    2. Central Hospital of Xinyang City, Xinyang 464000, China;
    3. College of Life Science, Xinyang Normal University, Xinyang 464000, China;
    4. College of Chemistry and Molecular Science, Wuhan University, Wuhan
    430072, China
  • Received:2006-01-26 Revised:1900-01-01 Online:2006-07-10 Published:2006-07-10
  • Contact: LIU Yan-Ming

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被引次数

20

摘要： 本文首次报道了人血清免疫反应后直接进样并利用毛细管电泳分离化学发光灵敏检测AFP的新方法, 检测结果与医院使用的全自动微粒子化学发光仪检测结果吻合.

关键词: 毛细管电泳, 免疫分析, 化学发光检测, 甲胎蛋白

Abstract: A novel detection method of a tumor marker alpha-fetoprotein(AFP) in human sera via capillary electrophoresis immunoassay coupled with chemiluminescence detection(CEIACL) was established. After a simple non-competitive immunoassay procedure, seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by p\|iodophenol.

Key words: Capillary electrophoresis, Immunoassay, Chemiluminescence detection, alpha-Fetoprotein

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