高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (7): 1606.doi: 10.7503/cjcu20130077

• 分析化学 • 上一篇    下一篇

β-环糊精联吡啶钌衍生物与维生素包络作用的荧光光谱分析

郑珊珊, 陈红, 祁彦涛, 王秀华, 樊华军, 赵莹莹, 张帆, 杨帆, 汤杰, 何品刚   

  1. 华东师范大学化学系, 上海 200062
  • 收稿日期:2013-01-22 出版日期:2013-07-10 发布日期:2013-06-21
  • 通讯作者: 何品刚, 男, 博士, 教授, 博士生导师, 主要从事生物传感器的研究. E-mail: pghe@chem.ecnu.edu.cn E-mail:pghe@chem.ecnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21075042)资助.

Analysis of Binding Properties Between β-Cyclodextrin Bipyridine-ruthenium Derivative and Vitamins by Their Fluorescence Spectra

ZHENG Shan-Shan, CHEN Hong, QI Yan-Tao, WANG Xiu-Hua, FAN Hua-Jun, ZHAO Ying-Ying, ZHANG Fan, YANG Fan, TANG Jie, HE Pin-Gang   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • Received:2013-01-22 Online:2013-07-10 Published:2013-06-21
  • Contact: Pingang E-mail:pghe@chem.ecnu.edu.cn

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摘要：

合成了一种新型的β-环糊精联吡啶钌衍生物[β-CD-Ru(bpy)3], 采用分子荧光光谱法研究了该衍生物对一系列维生素的包络作用. β-CD-Ru(bpy)3的结构中含有发光活性基团联吡啶钌中心, 因此具有良好的荧光特性. 当加入与β-环糊精有主客体识别作用的维生素时, β-CD-Ru(bpy)3与维生素之间发生能量转移从而导致主体分子发生荧光猝灭. 采用Benesi-Hildebrand方程, 根据其猝灭值计算了该衍生物与各种维生素的包络常数. 实验结果表明, 维生素与β-CD-Ru(bpy)3包络作用大小顺序为维生素B12>维生素K3>维生素C>维生素A.

关键词: β-环糊精联吡啶钌衍生物, 维生素, 荧光光谱, 包络作用

Abstract:

This paper presents a research of the binding interaction of a new synthetized β-cyclodextrin bipyridine-ruthenium derivative[β-CD-Ru(bpy)3] with a series of vitamins using fluorescent spectrometry. The performance of fluorescence emission of β-CD-Ru(bpy)3 was excellent due to the existence of the center of bipyridine-ruthenium. Based on the effect of molecular recognition between β-cyclodextrin and guest molecule, the fluorescence quenching occured because of the energy transfer between the β-CD-Ru(bpy)3 and the vitamin when a kind of vitamin was added. According to the quantitative value of fluorescence quenching, the binding constants of the new derivative with respect to various vitamins were determined by Benesi-Hildebrand Equation. The results show that the binding capacities of the vitamins with β-CD-Ru(bpy)3 were in a descending sequence of VB12>VK3>VC>VA.

Key words: β-Cyclodextrin bipyridine-ruthenium derivative, Vitamin, Fluorescence spectra, Binding interaction

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