高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (2): 331.doi: 10.7503/cjcu20120467

• 分析化学 • 上一篇    下一篇

红车轴草总异黄酮成分DNA结合剂的超滤质谱筛选

马蕾1,2, 王兆伏1, 陈丽娜1,2, 宋凤瑞1, 刘志强1, 刘淑莹1   

  1. 1. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
    2. 中国科学院大学, 北京 100049
  • 收稿日期:2012-05-14 出版日期:2013-02-10 发布日期:2013-01-21
  • 通讯作者: 刘志强,男,博士,研究员,博士生导师,主要从事天然药物化学与有机质谱学研究.E-mail:liuzq@ciac.jl.cn E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 21073178, 21175128)资助.

Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MSn

MA Lei1,2, WANG Zhao-Fu1, CHEN Li-Na1,2, SONG Feng-Rui1, LIU Zhi-Qiang1, LIU Shu-Ying1   

  1. 1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-05-14 Online:2013-02-10 Published:2013-01-21

PDF (PC)

被引次数

10

摘要：

采用离心超滤和液相色谱-质谱联用的方法, 从红车轴草异黄酮提取物中筛选DNA结合剂. 结果表明, 红车轴草中10种异黄酮成分与DNA具有不同的结合能力. 采用液相色谱-串联质谱联用技术对其结构进行了鉴定, DNA结合能力较强的5种化合物分别是德鸢尾素-4'-O-β-D-葡萄糖苷、 德鸢尾素、 黄豆黄葡萄糖苷、 红车轴草素和鹰嘴豆牙素A. 为从中药提取物等复杂体系筛选并鉴定DNA结合剂建立了快速的超滤质谱平台.

关键词: 红车轴草, DNA, 离心超滤, 液质联用

Abstract:

Centrifugal ultrafiltration and LC-MS methods were combined to screen DNA-binding agents from traditional Chinese medicine red clover isoflavone extracts. The results show that 10 red clover isoflavones have different binding degrees with DNA. The sample was analyzed by LC-MSn for identifying structures. Among these flavones studied, the strongest five potential DNA-binding agents are irilone-4'-O-β-D-glucoside, glycitin, pratensein, irilone and biochanin A, respectively. A rapid centrifugal ultrafiltration-LC-MS platform for screening and identification of DNA binders from the herbal extract and other complex system was established.

Key words: Trifolium pratense L., DNA, Centrifugal ultrafiltration, LC-MS

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