高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (7): 1492.

• 研究论文 • 上一篇    下一篇

Pd(Ⅱ)与色氨酸、酪氨酸及苯丙氨酸相互作用的荧光光谱

许倩影, 刘忠芳, 胡小莉, 孔玲, 刘绍璞   

  1. 发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
  • 收稿日期:2010-09-14 修回日期:2010-10-17 出版日期:2011-07-10 发布日期:2011-06-02
  • 通讯作者: 刘绍璞 E-mail:liusp@swu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20875078)资助.

Fluorescence Spectroscopy Study on the Interaction Between Pd(Ⅱ) and Tryptophan, Tyrosine and Phenylalanine

XU Qian-Ying, LIU Zhong-Fang, HU Xiao-Li, KONG Ling, LIU Shao-Pu*   

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
  • Received:2010-09-14 Revised:2010-10-17 Online:2011-07-10 Published:2011-06-02
  • Contact: LIU Shao-Pu E-mail:liusp@swu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20875078)资助.

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被引次数

25

摘要： 当Pd(Ⅱ)与色氨酸(Trp)、酪氨酸(Tyr)及苯丙氨酸(Phe)等芳香族氨基酸相互作用时，能观察到3种氨基酸的荧光均发生猝灭. 从吸收光谱的变化，温度对猝灭作用的影响以及猝灭常数Ksv，可以判定荧光猝灭作用是由于Pd(Ⅱ)与上述氨基酸形成基态配合物而导致的静态猝灭过程. 并认为在一定浓度的Cl-存在下，Pd(Ⅱ)与氨基酸分别以N, N配位和N, O配位形成以下混配型三元配合物Pd(HR)Cl2 (Trp和Phe体系)和Pd(H2R)Cl2(Tyr体系)，并推测了配合物相应的结构. 该荧光猝灭体系不仅可用于研究钯(Ⅱ)与上述芳香族氨基酸的相互作用，也可成为以氨基酸(特别是Trp)作探针高灵敏荧光猝灭法测定钯的基础.

关键词: 荧光猝灭, Pd(Ⅱ), 色氨酸, 酪氨酸, 苯丙氨酸

Abstract: When Pd(Ⅱ)reacted with tryptophan(Trp), tyrosine(Tyr) and phenylalanine(Phe), the fluorescence of the three aromatic amino acids was all quenched. From the change of absorption spectrum, the effect of the temperature and the quenching constant Ksv, this procedure was judged to be static quenching on account of Pd(Ⅱ)interacting with the above amino acids to form groundstate coordination compounds. We also thought that in present of certain concentration of Cl-, Pd(Ⅱ)coordinated with amino acids respectively by N, N and N, O to form mixed-type ternary complexes Pd(HR)Cl2 (Trp and Phe system) and Pd(H2R)Cl2(Tyr system), the corresponding structures were presumed. This fluorescence system could not only be used in the study on the interaction between Pd(Ⅱ)and above aromatic amino acids, but also be foundation of fluorescence quenching method determining Pd(Ⅱ)using amino acid (especially Trp ) as probe.

Key words: fluorescence quenching, Pd(Ⅱ), tryptophan, tyrosine, phenylalanine

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