高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (12): 2748.

• 研究论文 • 上一篇    下一篇

d(C4)相关序列形成四分子i-Motif结构的电喷雾质谱研究

秦玉娇1, 魏士刚1, 王晓录1, 杨帆2, 汪兵1, 国新华1   

  1. 1. 吉林大学化学学院, 长春 130012;
    2. 松辽流域水资源保护局, 长春 130021
  • 收稿日期:2011-06-24 修回日期:2011-08-17 出版日期:2011-12-10 发布日期:2011-11-25
  • 通讯作者: 国新华 E-mail:guoxh@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号： 20575066, 20975044)资助.

Electrospray Ionization Mass Spectrometry Studies of Tetramolecular i-Motif Structures Formed by d(C4) Related Sequences

QIN Yu-Jiao1, WEI Shi-Gang1, WANG Xiao-Lu1, YANG Fan2, WANG Bing1, GUO Xin-Hua1*   

  1. 1. College of Chemistry, Jilin University, Changchun 130012, China;
    2. Songliao River Basin Water Resource Protection Bureau, Changchun 130021, China
  • Received:2011-06-24 Revised:2011-08-17 Online:2011-12-10 Published:2011-11-25
  • Contact: GUO Xin-Hua E-mail:guoxh@jlu.edu.cn
  • Supported by:

    国家自然科学基金(批准号： 20575066, 20975044)资助.

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被引次数

17

摘要： 设计了与富含胞嘧啶(C)的DNA序列d(C4)相关的DNA序列d(C4), d(TC4), d(AC4), d(T2C4), d(A2C4), d(C4T), d(C4A)和d(TC4T); 采用电喷雾质谱测定发现这些序列形成四分子非共价复合物离子, 根据离子的相对丰度可确定形成四链i-motif结构的数量和可能性; 同时考察了腺嘌呤(A)和胸腺嘧啶(T)在d(C4)序列的5'和3'端对其形成四分子i-motif结构的影响. 结果表明, 在d(C4)的5'端增加A碱基或T碱基更易形成四分子复合物; 5'端含T碱基比含A碱基更利于形成四分子复合物; 而在d(C4)序列中增加2个A碱基或T碱基比增加相应的单个碱基形成了更高丰度的四分子离子峰.

关键词: DNA i-motif 结构, 电喷雾质谱, d(C4)相关序列, 非共价相互作用

Abstract: A series of cytosine-rich DNA sequence d(C4) related DNA strands, including d(C4), d(TC4), d(AC4), d(T2C4), d(A2C4), d(C4T), d(C4A) and d(TC4T), was designed in this study, and the tetramolecular ions formed by the sequences were measured with electrospray ionization mass spectrometry. The goal is to obtain the information on the formation of the tetramolecular i-motif influenced by adenine(A) and thymine(T) that present at 5' and 3' ends of d(C4). The results showed that when A base or T base was added at 5' end of d(C4), it is easier for the sequence to form tetramolecular ion, especially for thymine, when adding two A or T bases to d(C4), the sequence generates higher abundant of the tetramolecualr ion.

Key words: DNA i-motif structure, Electrospray ionization mass spectrometry, d(C4) related sequence, Non-covalent interaction

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