关键词: 阳虚体质, 核磁共振, 代谢组学

Abstract: Nuclear magnetic resonance(NMR)-based metabonomics was applied to study the yang-deficiency constitution by analyzing metabolic profiling of serum and urine. The purpose of this study is to determine an array of characteristic metabolites in serum and urine samples from yang-deficiency constitution person and then to interpret these metabolites in possible metabolic pathway.  Serum and urine samples from 30 yang-deficiency constitution person with 30 balanced constitution person were collected. Differences in endogenous metabolites were detected on serum and urine samples from the yang-deficiency constitution group and control group using multivariate statistical analysis. Compared with the balanced constitution, the contents of lactate, very low density lipoprotein(VLDL)/low density lipoprotein(LDL), N-cetyl glycoprotein(NAc), fatty acid(FA), unsaturated fatty acid(UFA) are lower while the contents of glutamine, glucose, phosphatidylcholine(PtdCho), high density lipoprotein(HDL) are higher in serum of yang-deficiency constitution, and the content of creatinine is lower while the contents of lactate, DMA, citrate, hippurate are higher in urine of yang-deficiency constitution. The biomarkers supply new ways for the individual differences from the point of view of metabonomics. Yang-deficiency constitution has some metabolism disorder of energy, lipid and glucose, and also  has changes of organs functions.

Key words: Yang-deficiency constitution, Nuclear magnetic resonance(NMR), Metabonomics