高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

茶多酚锰-壳聚糖微球的制备、控释和诱导肝癌细胞凋亡的研究

黄河宁1,2, 李程1, 谢笠升1, 黄河清1,3   

    1. 厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室, 厦门 361005;
    2. 三明学院化学与生物工程系, 三明 365004;
    3. 厦门大学化学化工学院固体表面物理化学国家重点实验室, 福建省化学生物学重点实验室, 厦门 361005
  • 收稿日期:2007-08-07 修回日期:1900-01-01 出版日期:2008-08-10 发布日期:2008-08-10
  • 通讯作者: 黄河清

Preparation, Release-control and Cell Apoptosis of Liver Cancer in the Tea Polyphenol Manganese Loaded on a Chitosan Microparticles

HUANG He-Ning1,2, LI Cheng1, XIE Li-Sheng1, HUANG He-Qing1,3*   

    1. The Key Laboratory of MOE for Cell Biology and Tumor Cell Engineering of Ministry of Education, School of Life Sciences, Xiamen University, Xiamen 361005, China;
    2. Department of Chemical and Biological Engineering, College of Sanming, Sanming 365004, China;
    3. State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2007-08-07 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10
  • Contact: HUANG He-Qing

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被引次数

5

摘要： 选用壳聚糖为微米粒包被材料, 制备茶多酚锰(Tea Polyphenol Manganese, TPMn)-壳聚糖微球. 用荧光显微技术研究了TPMn-壳聚糖微球的荧光特性, 用扫描和透射电子显微镜证实TPMn-壳聚糖微球尺寸和分布规律. RP-HPLC定量分析TPMn-壳聚糖微球包封率为68%, 符合微米级微粒控释药物包封率的要求. 动力学研究结果表明, 茶多酚(TP)-壳聚糖和TPMn-壳聚糖的微球均有控释TP的能力, 控释时间高达40 h以上, 但前者释放速率稍快于后者. TPMn和TPMn-壳聚糖微球均能诱导肝癌细胞凋亡, 但TPMn-壳聚糖微球诱导肿瘤细胞的凋亡速率稍高于TPM. 实验结果证实, 以TPMn-壳聚糖微球方式控释TPMn有利于提高诱导肿瘤细胞凋亡速率. TPMn-壳聚糖微球具有研发成注射型抗肿瘤新药的可能性.

关键词: 茶多酚锰-壳聚糖微球, 理化特性, 控释, 肝癌细胞, 凋亡

Abstract: The chitosan as a wrapping material was selected to prepare a microparticles of tea polyphenol manganese-chitosan(TPMn). Fluorescence characteristics of TPMn-chitosan microparticles was revealed by fluorescence microscopy. Size and distribution orderliness of TPMn-chitosan microparticles was further approved by the scanning electron microscopy and the transmission electron microscopy, respectively. The envelopment ratio of TPMn-chitosan microparticles was calculated to be 68% by RE-HPLC approach, which is in accordance with the ratio request of release-controlled drug at the micron level of microparticles. The results of kinetic studies show that two chitosan microparticles loaded on both tea polyphenol(TP) and TPMn, respectively, have capacity for controlling-release TP for exceeding 40 h, but this rate in TP microparticles was somewhat quicker than that of TPMn microparticles. Even so, both miscroparticles have capacity for inducing cells of liver cancer apoptosis, but this apoptosis rate by TPMn-chitosan microparticles was somewhat higher than that by TP-chitosan microparticles. The experimental results prove that the TPMn-chitosan microparticles was propitious to release and control TPMn for enhancing the apoptosis rate of tumour cell induced. TPMn-chitosan microparticles would have a potential for developing a new injecting drug for antitumor.

Key words: TPMn-chitosan microparticles, Characteristics of physical chemistry, Controlling-release, Cell of liver cancer, Apotosis

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