高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

温敏性壳聚糖共聚膜的制备与细胞吸附/脱附行为

陈莉1, 李世庚1, 肖飞1, 张爱华2, 姚康德2   

    1. 天津市改性与功能纤维重点实验室, 天津工业大学材料科学与化学工程学院, 天津 300160;
    2. 天津大学高分子材料研究所, 天津 300072
  • 收稿日期:2007-09-11 修回日期:1900-01-01 出版日期:2008-05-10 发布日期:2008-05-10
  • 通讯作者: 陈莉

Preparation of Thermoresponsive Chitosan Copolymer and Its Cytocompatibility and Detachability

CHEN Li1*, LI Shi-Geng1, XIAO Fei1, ZHANG Ai-Hua2, YAO Kang-De2   

    1. Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Materials Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China;
    2. Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China
  • Received:2007-09-11 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
  • Contact: CHEN Li

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被引次数

18

摘要： 将丙烯酸(AAc)与壳聚糖(CS)反应, 合成了壳聚糖大单体(CS-AAc), 再用CS-AAc与N-异丙基丙烯酰胺(NIPAAm)共聚, 制备P(CS-AAc-NIPAAm)共聚物. 通过红外光谱和X射线光电子能谱等分析证实了产物的结构和组成. 对P(CS-AAc-NIPAAm)共聚膜的动态接触角及对细胞的吸附与脱附行为研究发现, 共聚膜表现出良好的温度敏感性, 其表面成功地种植了成纤维细胞(L929). 当环境温度降低后, 共聚膜表面细胞自动脱附, 从而避免了使用酶解法脱附细胞造成的细胞功能损伤.

关键词: 聚N-异丙基丙烯酰胺, 壳聚糖, 温度敏感性, 细胞培养, 细胞脱附

Abstract: In this study, Chitosan(CS) based macromer was used to modify NIPAAm to make a novel temperature-responsive film for cell adhesion and detachment. CS was coupled with acrylic acid(AAc) to prepare a macromer(CS-AAc). Then, it was copolymerized with N-isopropylacrylamide(NIPAAm) to synthesize a P(CS-AAc-NIPAAm) film. The novel copolymer obtained was characterized via FTIR and XPS. There was thermo-response for the film against water, and this phenomenon was coincided with the data of dynamic contact angle against water. Moreover, the cytocompatibility of the film was investigated with fibroblast culture in vitro. The result reveal that the film exhibited a good cytocompatibility, when the temperature declined, it had the cell detachability.

Key words: PNIPAAm, Chitosan, Temperature sensitivity, Cell culture, Cell detachment

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