高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (4): 612.

• 研究论文 • 上一篇    下一篇

全二维气相色谱/飞行时间质谱用于烟叶中酸性成分的分离与分析

李海锋1,3, 路鑫1, 鹿洪亮2, 马晨菲1,  朱书奎1,  孔宏伟1,  赵明月2 , 谢剑平2,  叶耀睿1, 牛森3,  许国旺1   

  1. 1. 中国科学院大连化学物理研究所, 国家色谱研究分析中心, 大连 116023;
    2. 郑州烟草研究院, 郑州 450000; 3. 沈阳农业大学植物保护学院, 沈阳 110161
  • 收稿日期:2005-07-04 出版日期:2006-04-10 发布日期:2006-04-10
  • 通讯作者: 许国旺(1963年出生)， 男， 研究员， 博士生导师， 从事高分辨分离分析的方法学研究. E-mail: xugw@dicp.ac.cn
  • 基金资助:

    国家自然科学杰出青年基金(批准号: 20425516)和国家烟草专卖局科研基金资助

Separation and Analysis of Constituents of Acidic Compounds in Tobacco Leaf by Using Comprehensive Two-dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

 LI Hai-Feng 1,3, LU Xin1, LU Hong-Liang 2, MA Chen-Fei1, ZHU Shu-Kui1,  KONG Hong-Wei1,ZHAO Ming-Yue2, XIE Jian-Ping2, YE Yao-Rui1, NIU Sen3, XU Guo-Wang1*   

  1. 1. National Chromatographic R & A Center, Dalian Institute of Chemical Physics,   Chinese Academy of Sciences, Dalian 116023, China; 2.  Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, China; 3. Plant Protection Department, Shenyang Agriculture University, Shenyang 110161, China
  • Received:2005-07-04 Online:2006-04-10 Published:2006-04-10
  • Contact: XU Guo-Wang, E-mail: xugw@dicp.ac.cn

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摘要：

建立了烟叶中酸性化合物(挥发性、 半挥发性羧酸类和酚类)组成研究的全二维气相色谱/飞行时间质谱(GC×GC-TOFMS)分析方法, 并用此方法对香料烟中的酸性化合物进行了表征. 用同时蒸馏萃取样品的前处理方法, 采用TOFMS谱图库检索结合全二维特有的包含结构信息的二维谱图, 通过族分离和结构谱图, 鉴定出了香料烟中143种挥发性及半挥发性酸性组分, 包括10种酸酐和呋喃二酮, 43种有机酸和90种酚类化合物. 同时对不同类别的化合物在二维气相色谱上的分布模式进行了研究. 结果表明, 全二维气相色谱飞行时间质谱的高分辨率非常适合于烟叶这类复杂体系的分离分析.

关键词: 烟叶; 酸性化合物; 全二维气相色谱(GC×GC)； 飞行时间质谱(TOFMS)

Abstract:

Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry(GC×GC-TOFMS) was applied to the  characterization of volatile and semi-volatile acidic compounds in oriental tobacco leaf. Simultaneous distillation extraction(SDE) was used as the sample extraction method. Two types of column sets were compared, and appropriate GC×GC separation conditions were suggested. The separation behavior on two-dimensimal plot of different kinds of compounds were investigated. TOFMS library search combining with  structured chromatogram of GC×GC was used to identify the acidic compounds in tobacco leaf. A total of 143 acidic compounds were tentatively identified, including 10 acid anhydrides, 43 organic acids and 90 phenolic compounds. The results show that GC×GC-TOFMS method is suitable for separation and identification of complex sample.

Key words: Tobacco leaf; Acidic compounds; Comprehensive two dimensional gas chromatography(GC×GC); Time of flight mass spectrometry(TOFMS)

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