高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (11): 2411.doi: 10.7503/cjcu20120290

• 分析化学 • 上一篇    下一篇

牛血清白蛋白修饰的近红外荧光纳米金顺磁标记分子探针

何微娜1, 涂熹娟1,2, 郭祥群1   

  1. 1. 福建省化学生物学重点实验室, 厦门大学化学化工学院化学系, 分析科学重点实验室, 厦门 361005;
    2. 福建农林大学蜂学学院, 福州 350002
  • 收稿日期:2012-04-01 出版日期:2012-11-10 发布日期:2012-10-15
  • 通讯作者: 郭祥群,女,博士,教授,博士生导师,主要从事分子发光分析研究.E-mail:xqguo@xmu.edu.cn E-mail:xqguo@xmu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20835005, 20975086, J1030415)资助.

Spin-Labeled BSA-modified Near-infrared Fluorescent Gold Nanoparticles

HE Wei-Na1, TU Xi-Juan1,2, GUO Xiang-Qun1   

  1. 1. Key Laboratory for Chemical Biology of Fujian Province, Department of Chemistry and Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
    2. Bee Science College, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2012-04-01 Online:2012-11-10 Published:2012-10-15

PDF (PC)

被引次数

4

摘要：

以光稳定性良好、 Stokes位移大且可近红外发射的谷胱甘肽包裹纳米金(GSH-AuNPs)为发光载体, 以4-氨基-2,2,6,6-四甲基哌啶氮氧自由基(4-NH2-TEMPO)作为顺磁标记基团, 对构建发光-顺磁双模式传感分子探针进行了研究; 以牛血清白蛋白(BSA)为表面修饰剂, 通过调节荧光纳米金的表面状态, 改善顺磁标记微环境, 获得了基于顺磁基团识别诱导信号传导的荧光-顺磁双模式响应型分子探针. 顺磁标记BSA修饰GSH-AuNPs形成弱荧光-强顺磁复合物(GSH-AuNPs@BSA-TEMPO), 复合物中顺磁基团TEMPO经抗坏血酸还原后呈现出荧光增强和顺磁信号减弱现象, 表现出对抗坏血酸浓度相关的荧光Off-on与顺磁On-off的双模式响应.

关键词: 近红外荧光纳米金, 分子探针, 氮氧自由基, 顺磁标记

Abstract:

Bovine serum albumin(BSA)-modified spin-labeled near-infrared fluorescence gold nanoparticles as bimodal molecular probe was designed and synthesized. Glutathione capped gold nanoparticles(GSH-AuNPs) was synthesized and modified by BSA, the BSA modified fluorescent gold nanoparticles conjugates(GSH-AuNPs@BSA) was spin-labeled by paramagnetic nitroxide radical and gave a spin-labeled conjugate GSH-AuNPs@BSA-TEMPO. The introduction of BSA to the conjugate was to further modify the surface of GSH-AuNPs in order to enhance the intrinsic fluorescence of GSH-AuNPs, and to facilitate the spin labeling. GSH-AuNPs@BSA-TEMPO itself was a weak fluorescent conjugate and showed strong ESR signal properties. Reducing the paramagnetic functional group TEMPO in the as-prepared conjugates to its counterpart hydroxylamine by ascorbic acid, both Off-on fluorescence increase and On-off ESR decrease are observed, showing concentration related bimodal response to ascorbic acid(AA).)

Key words: Near-infrared fluorescent AuNPs, Molecular probe, Nitroxide free radical, Spin-labeled

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