高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (08): 1676-1680.doi: 10.3969/j.issn.0251-0790.2012.08.008

• 分析化学 • 上一篇    下一篇

基于单壁碳纳米管-DNA酶复合结构的电化学生物传感器

封科军1, 姚艳玲1, 沈国励2, 俞汝勤2   

  1. 1. 惠州学院化学工程系, 惠州 516007;
    2. 湖南大学化学化工学院, 化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2012-03-29 出版日期:2012-08-10 发布日期:2012-08-10
  • 通讯作者: 封科军, 女, 博士, 讲师, 主要从事化学与生物传感及其在生化、 环境及材料分析中的应用研究. E-mail: hndxfkj@hotmail.com E-mail:hndxfkj@hotmail.com
  • 基金资助:

    国家自然科学基金重点项目(批准号: 21035001)和广东省自然科学基金项目(批准号: 10451601501006188)资助.

Single-walled Carbon Nanotube/DNAzyme Complex-based Elctrochemical Biosensors

FENG Ke-Jun1, YAO Yan-Ling1, SHEN Guo-Li2, YU Ru-Qin2   

  1. 1. Department of Chemical Engineering, Huizhou University, Huizhou 516007, China;
    2. State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
  • Received:2012-03-29 Online:2012-08-10 Published:2012-08-10

摘要: 结合DNA酶优异的氧化还原催化特性和碳纳米管的电化学特性, 制备了单壁碳纳米管-DNA酶复合材料, 并通过壳聚糖将其固定到玻碳电极表面构建了电化学生物传感界面. 研究了单壁碳纳米管-DNA酶复合结构的氧化还原反应催化特性, 并以此为传感平台构建了葡萄糖氧化酶电化学生物传感器. 结果表明, 单壁碳纳米管-DNA酶复合材料修饰的电极对过氧化氢的响应具有较宽的线性范围(5×10-6~1×10-2 mol/L)和良好的检测灵敏度(检出限为1×10-6 mol/L). 采用制备的葡萄糖氧化酶传感器实现了对葡萄糖的快速灵敏检测.

关键词: 碳纳米管, DNA酶, 电化学生物传感器, 纳米复合材料, 催化性能

Abstract: Taking advantage of the excellent catalytic redox activity andthe electrochemical property of DNAzyme and carbon nanotubes, a novel nano-complex of single-walled carbon nanotubes(SWNTs)/DNAzyme was prepared, and the obtained nano-complex was finally immobilized on the surface of glass carbon electrode(GCE) with the assistance of chitosan to fabricate electrochemical biosensing interface. The catalytic property of the SWNTs/DNAzyme complex was explored, and a glucose oxidase biosensor was then prepared based on the fabricated electrochemical biosensing platform. The SWNTs/DNAzyme complex-based biosensing platform can direct response to hydrogen peroxide at low potential of -0.24 V with high sensitivity(the detection limit is 1?10-6 mol/L) and wide linear range(5?10-6-1?10-2 mol/L), and the prepared glucose oxidase biosensor was used to fast and sensitive detection of glucose.

Key words: Carbon nanotube, DNAzyme, Electrochemical biosensor, Nano-complex, Catalytic property

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