高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 1035.

• 研究论文 • 上一篇    下一篇

自组装膜上细胞色素c的电化学石英晶体微天平实时表征和定量检测

李金花, 胡劲波, 李启隆   

  1. 北京师范大学化学系, 北京100875
  • 收稿日期:2004-10-20 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 胡劲波(1965年出生),女,副教授,主要从事生物电分析研究.E-mail:hujingbo@bnu.edu.cn E-mail:hujingbo@bnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20275007)资助.

Real-time Characterization and Quantitative Detection of Cytochrome c Based on SAMs with Electrochemical Quartz Crystal Microbalance

LI Jin-Hua, HU Jin-Bo, LI Qi-Long   

  1. Department of Chemistry, Beijing Normal University, Beijing 100875, China
  • Received:2004-10-20 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

13

摘要： 采用电化学石英晶体微天平(EQCM)实时表征和定量检测细胞色素c(Cytc).在压电石英晶振表面上自组装巯基十一酸(MUA)单层膜,以盐酸1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)活化羧基,将Cytc共价固化到电极表面.EQCM实时监测了MUA的自组装和Cytc的固化过程,测定了二者在电极表面的覆盖度和Cytc的固化量.结果表明,Cytc在0.03～3.00μmol/L浓度范围内呈线性变化,检测限可达到1.19×10-9mol/L.

关键词: 自组装膜, 细胞色素c(Cytc), 电化学石英晶体微天平(EQCM)

Abstract: Electrochemical quartz crystal microbalance(EQCM), which is capable of sensitive measurement for mass and electrochemical signals, was employed to study cytochrome c. Self-assembled monolays(SAMs) with carboxy groups were first coated on the gold electrode of a quartz crystal resonator by adsorption of ω-mercaptoundecanoic acid(MUA), cytochrome c was then covalently attached to the surface via N-ethyl-N'- carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). The processes for self-assembly of MUA and immobilization of cytochrome c were both real-time characterized. And the surface coverage densities of MUA and cytochrome c were estimated to be 3.73×10-9 and 7.31×10-11 mol/cm2, respectively. The amount of cytochrome c was found to be proportional to the frequency changes between 0.03 μmol/L and 3.0 μmol/L, with a regression equation Δf(Hz)=50.19 c(Cyt C)+67.07(r2=0.998), and the limit of detection of 1.19×10-9 mol/L was also attained.

Key words: Self-assembled monolayers(SAMs), Cytochrome c, Electrochemical quartz crystal microbalance(EQCM)

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