高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 1030.

• 研究论文 • 上一篇    下一篇

荧光性自组装双层膜的制备及其性能研究

孙向英, 翁文婷   

  1. 华侨大学材料科学与工程学院, 泉州362021
  • 收稿日期:2004-04-20 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 孙向英(1965年出生),女,博士,教授,主要从事光化学及电化学分析研究.E-mail:liumy@hqu.edu.cn E-mail:liumy@hqu.edu.cn
  • 基金资助:

    国务院侨务办公室科研基金(批准号:03QZR12);福建省自然科学基金(批准号:D0410019)资助.

Preparation and Properties of Fluorescent Self-assembled Bilayer Membrane

SUN Xiang-Ying, WENG Wen-Ting   

  1. College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China
  • Received:2004-04-20 Online:2005-06-10 Published:2005-06-10

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被引次数

32

摘要： 借助Au—S化学键的作用,在金基底上组装DL-半胱氨酸,利用DL-半胱氨酸与1-萘胺乙酸(NAA)的静电吸引作用在金表面间接组装荧光试剂NAA,从而构建了双层自组装膜NAA/Cys/Au.该自组装膜有较强的荧光信号,能被Cu2+猝灭,并具有较好的可逆再生性能,可用于超痕量铜离子的界面荧光测定,对Cu2+的检出下限为7.87×10-11mol/L.同时采用电化学、荧光光谱及电子能谱等方法表征自组装膜的结构,并采用电化学阻抗谱技术和循环伏安法研究自组装膜在K3[Fe(CN)6]/K4[Fe(CN)6]溶液中的电化学行为研究.结果表明,金表面组装的单层膜具有良好的“针孔”效应,组装上荧光试剂之后形成的无“针孔”缺陷的自组装双层膜对溶液与基底间的界面电子转移有强烈的阻碍作用.

关键词: 界面荧光, 自组装双层膜, 循环伏安, 电化学阻抗谱, 铜离子

Abstract: Fluorescent reagent 2-(1-naphthyl)aminoacetic acid(NAA) was assembled onto the gold surface via its electrostatic interaction with DL-cysteine(Cys) that was directly assembled on the gold surface via AuS bond. Strong fluorescence was observed from the NAA self-assembled bilayer membrane on the gold surface and was highly efficiently quenched by Cu2+ that was allowed for an extremely highly sensitive detection of Cu2+ with a detection limit of 7.87×10-11 mol/L. The fluorescence from NAA/Cys/Au can be easily the regenerated and therefore the present report showed a reusable method for immobilizing reagent in fabricating fluorescent chemosensors. The self-assembled monolayer and bilayer were primarily characterized by electrochemical method and electron energy spectrum. The electrochemical behavior of the SAMs on the gold electrode in the solution of (K3)/K4 was investigated by cyclic voltammetry and AC impedance spectroscopy. The result of the experiment shows that Cys/Au has an excellent pinhole property to enhance the interfacial electron transfer, when the NAA was assembled onto the electrode the pinhole-free properties block the interfacial electron transfer from redox couple in the solution to the substrate.

Key words: Interfacial fluorescence, Self-assembled bilayer membrane, Cyclic voltammetry, Electrochemical impedance, Copper ion

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