高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 841.

• 研究简报 • 上一篇    下一篇

以乙二胺为手臂分子制备的DNA修饰电极及其伏安性能

焦奎1, 徐桂云1,2, 张旭志1, 米春华1   

  1. 1. 青岛科技大学化学与分子工程学院, 青岛266042;
    2. 中国海洋大学化学与化工学院, 青岛266003
  • 收稿日期:2004-06-23 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 焦　奎(1945年出生),男,博士,教授,博士生导师,从事电分析化学研究.E-mail:kjiao@qust.edu.cn E-mail:kjiao@qust.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20375020)资助.

Preparation and Voltammetric Characterization of DNA Modified Electrode with Ethylenediamine as Arm Molecule

JIAO Kui1, XU Gui-Yun1,2, ZHANG Xu-Zhi1, MI Chun-Hua1   

  1. 1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
    2. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China
  • Received:2004-06-23 Online:2005-05-10 Published:2005-05-10

PDF (PC)

被引次数

38

关键词: 脱氧核糖核酸(DNA), 乙二胺, 共价固定, 伏安法, 亚甲基蓝

Abstract: Carboxyl was formed on the surface of glassy carbon electrode(GCE) by electrochemical oxidation. Ethylenediamine(En) was used as the arm molecule to link carboxyl with dsDNA using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS) as the activators to prepare dsDNA modified electrode(dsDNA/En/GCE). It was shown that dsDNA could be covalently immobilized on the surface of GCE. ssDNA modified electrode(ssDNA/En/GCE) was obtained via the thermal denaturation of dsDNA/En/GCE. The dsDNA/En/GCE and ssDNA/En/GCE were characterized by voltammetry with methylene blue(MB) as the indicator. The results indicated that the currents of the redox peaks of MB at ssDNA/En/GCE were larger than those at dsDNA/En/GCE, and the currents of the redox peaks at En/GCE were the smallest. The peak-currents of MB at the DNA modified electrode had good reproducibility after multi-denaturation and hybridization cycles.

Key words: DNA, Ethylenediamine, Covalent immobilization, Voltammetry, Methylene blue

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