高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 835.

• 研究快报 • 上一篇    下一篇

牛血清白蛋白手性毛细管整体柱的制备及对映体的分离

严丽娟1,2, 张庆合1,3, 张维冰1, 冯钰錡2, 张丽华1, 李彤1,3, 张玉奎1   

  1. 1. 中国科学院大连化学物理研究所, 国家色谱研究分析中心, 大连116023;
    2. 武汉大学化学与分子科学学院, 武汉430072;
    3. 大连依利特分析仪器有限公司, 大连116011
  • 收稿日期:2004-11-10 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 张庆合(1968年出生),男,博士,副教授,主要从事分离、分析新技术与新材料的研究.E-mail:zhangqinghe@mail.china.com E-mail:zhangqinghe@mail.china.com
  • 基金资助:

    国家自然科学基金(批准号:20105006)资助.

Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography

YAN Li-Juan1,2, ZHANG Qing-He1,3, ZHANG Wei-Bing1, FENG Yu-Qi2, ZHANG Li-Hua1, LI Tong1,3, ZHANG Yu-Kui1   

  1. 1. National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
    2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
    3. Dalian Elite Analytical Instruments Co. Ltd., Dalian 116011, China
  • Received:2004-11-10 Online:2005-05-10 Published:2005-05-10

关键词: 毛细管电色谱, 整体柱, 溶胶-凝胶法, 手性固定相, 牛血清白蛋白

Abstract: A silica-bonded bovine serum albumin(BSA) chiral monolithic stationary phase for capillary electrochromatography(CEC) was introduced. An inorganic-organic hybrid monolithic column was firstly prepared by sol-gel chemistry with homogeneously distributed aminopropyl groups throughout the silica matrix. Then the chiral stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde. The effects of pH value and concentration of phosphate buffer on the separation of D,L-tryptophan were investigated. The separation factor of D,L-tryptophan reached 3.37 on CEC mode.

Key words: Capillary electrochromatography(CEC), Monolithic column, Sol-gel, Chiral stationary phases(CSPs), Bovine serum albumin(BSA)

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