高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (12): 2267.

• 研究简报 • 上一篇    下一篇

N-甲基吡咯类聚酰胺的合成及其与DNA的相互作用

李钦玲1,2,周江1,刘丹1,袁谷1   

  1. 1.北京大学化学学院化学生物学系生物有机与分子工程教育部重点实验室,北京100871;
    2.青海民族学院化学系,西宁810007
  • 收稿日期:2004-11-11 出版日期:1905-03-14 发布日期:1905-03-14
  • 通讯作者: 袁谷(1953年出生),男,博士,教授,博士生导师,主要从事化学生物学研究.E-mail:guyuan@pku.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20272005)资助.

Synthesis of Polyamides Containing N-Methylpyrrole Rings and Their Interaction with DNA

LI Qin-Ling1,2,ZHOU Jiang1,LIU Dan1,YUAN Gu1*   

  1. 1.Department of Chemical Biology,Key Laboratory of Bioorganic and Molecular Engineering of
    Education Ministry,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;
    2.Department of Chemistry,Qinghai University for Nationalities,Xining 810007,China
  • Received:2004-11-11 Online:1905-03-14 Published:1905-03-14
  • Contact: YUAN Gu;E-mail:guyuan@pku.edu.cn

被引次数

6

摘要：

本研究主要是设计并合成了3个含有4个N-甲基吡咯的聚酰胺化合物(PyPyPyPyβDp,PyPyPyPyγDp, NO2PyPyPyPyβDp),并使用ESI-MS法研究了其与DNA的相互作用.

关键词: 聚酰胺;电喷雾离子化质谱;DNA识别, 聚酰胺, 电喷雾离子化质谱, DNA识别

Abstract:

Polyamides containing N-methylpyrrole and N-methylimidazole amino acids have attracted considerable attention from biological chemists because they can permeate living cell membrane and have the potential to control specific gene expression.In this article,three polyamides containing N-methylpyrrole rings were synthesized by the chloroform reaction and the DCC/HOBT coupling reaction,and the interaction between the polyamides and DNA was investigated by electrospray ionization mass spectroscopy.The results of the experiments indicate that the designed polyamides can effectively bind the target DNA,and ESI mass spectrometry is an excellent tool for the analysis of the interactions between DNA and the polyamides.

Key words: Polyamides; Electrospray ionization mass spectroscopy; DNA recognition, Polyamides, Electrospray ionization mass spectroscopy, DNA recognition

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