高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (01): 176-181.doi: 10.3969/j.issn.0251-0790.2012.01.030

• 高分子化学 • 上一篇    下一篇

赖氨酸修饰聚酰胺-胺树枝状高分子的制备及性能

郭玲香, 高秋端   

  1. 东南大学化学化工学院, 南京 211189
  • 收稿日期:2011-02-28 出版日期:2012-01-10 发布日期:2011-12-20
  • 通讯作者: 郭玲香,女,博士,副教授,主要从事功能高分子材料研究.E-mail:glx_seu@yahoo.com.cn现在浙江雷可澳投资有限公司工作. E-mail:glx_seu@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号: 50873026, 20804009)和江苏省卫生厅重点科研课题(批准号: wkj2006-2-024)资助.

Synthesis and Performance of PAMAM-Lys Dendrimer

GUO Ling-Xiang, GAO Qiu-Duan   

  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
  • Received:2011-02-28 Online:2012-01-10 Published:2011-12-20

摘要: 通过液相合成法, 用L-赖氨酸(L-Lys)对4代聚酰胺-胺(4.0G PAMAM)进行表面修饰, 制备了新型的PAMAM-Lys树枝状高分子. 采用FTIR、 1H NMR、 元素分析和粒径分析等手段进行了结构表征. PAMAM-Lys的C, H, N元素含量分别为53.43%, 9.58%和24.29%, 端氨基测定值为2.18, 接近于理论计算值; 平均粒径约6.35 nm, 多分散系数约0.09. 应用透射电子显微镜和噻唑蓝四氮唑溴化物(MTT)比色法, 探讨了PAMAM和PAMAM-Lys树枝状高分子载体/质粒DNA复合物的形态及体外细胞毒性. 当最佳电荷比R+/-=4时, PAMAM-Lys与DNA形成复合物, 通过静电作用, 使DNA结构收缩, 质粒粒径介于50~100 nm之间, 分布较均匀, 形态规则; 作用于体外293T细胞时, PAMAM-Lys及其与DNA复合物的细胞毒性明显低于5代聚酰胺-胺(5.0G PAMAM). 研究结果表明, 制备的新型PAMAM-Lys树枝状高分子显著降低了高代数PAMAM树枝状高分子载体的细胞毒性, 具有良好的体外细胞相容性, 有望成为一种DNA疫苗的优良载体.

关键词: 树枝状高分子, 聚酰胺-胺, 赖氨酸, PAMAM-Lys复合物, 细胞毒性

Abstract: L-Lysine was used to modify 4.0G polyamidoaminec(PAMAM), and a new polyamidoamine derivative named PAMAM-Lys was synthesized via the liquid-phase synthesis. The structures of PAMAM-Lys were characterized by IR, 1H NMR, elementary analysis and granularmetric analysis. For PAMAM-Lys, the elemental analysis results of C, H and N were 53.43%, 9.58% and 24.29%, respectively, the end amino measured value was 2.18, close to the theoretical value. The mean grain size of PAMAM-Lys was about 6.35 nm and its particle size polydispersity was about 0.09. Microstructures of the composites were observed by TEM. The cytotoxicity tests in vitro transfected with PAMAM and PAMAM-Lys dendrimers were evaluated by Thiazolyl Blue Tetrazolium Bromide(MTT) assay. According to the observed results with TEM, the composite of PAMAM-Lys and plasmid DNA made the DNA structure shrink, the average grain diameter of plasmids ranged from 50 nm to 100 nm, plasmids had the even distribution and the regular morphology. On the basis of the MTT assay results, when acting on 293T in vitro, PAMAM-Lys and PAMAM-Lys/DNA had the lower cytotoxicity and the better biocompatibility than 5.0G PAMAM. It showed that PAMAM-Lys dendrimer had been synthesized successfully, the modification by lysine could reduce the cytotoxicity of PAMAM dendrimer significantly. PAMAM-Lys had the better biocompatibility in vitro and could improve the transfection efficiency for DNA, thus PAMAM-Lys would be a promising DNA vector in future.

Key words: Dendrimer, Polyamidoamine(PAMAM), L-Lysine, Composite of PAMAM-Lys, Cytotoxicity

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