全二维气相色谱/飞行时间质谱法在莪术挥发油组成分析中的应用

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (8): 1432.

全二维气相色谱/飞行时间质谱法在莪术挥发油组成分析中的应用

武建芳^1,2, 路鑫¹, 唐婉莹², 廉晓红³, 孔宏伟¹, 阮春海¹, 许国旺¹

1. 中国科学院大连化学物理研究所, 国家色谱研究分析中心, 大连116011;
2. 南京理工大学化工学院, 南京210094;
3. 大连医药科学研究所, 大连116011

收稿日期:2003-05-26 出版日期:2004-08-24 发布日期:2004-08-24
基金资助:
国家自然科学基金(批准号:20175028);中国科学院领域前沿创新基金(批准号:K2002A11)资助

Application of Comprehensive Two-dimensional Gas Chromatography/ Time-of-flight Mass Spectrometry to Analysis of Zedoary Volatile Oil

WU Jian-Fang^1,2, LU Xin¹, TANG Wan-Ying², LIAN Xiao-Hong³, KONG Hong-Wei¹, RUAN Chun-Hai¹, XU Guo-Wang¹

1. National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China;
2. College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China;
3. Dalian Institute of Medical and Pharmacetical Science, Dalian 116011, China

Received:2003-05-26 Online:2004-08-24 Published:2004-08-24

摘要/Abstract

摘要： 通过优化GC×GC的柱系统、温度程序和调制参数等色谱条件,建立了分析中药莪术挥发油组成的全二维气相色谱/飞行时间质谱(GC×GC/TOFMS)方法,实现了莪术挥发油的单个组分与族组分分析.采用所建立的GC×GC/TOFMS方法,鉴定出匹配度大于800的组分有249种,其中单萜18种,单萜含氧衍生物34种,倍半萜35种,倍半萜含氧衍生物37种,有69种组分的体积分数大于0.02%.

关键词: 挥发油, 莪术, 柱系统, 全二维气相色谱, 飞行时间质谱

Abstract: After optimizing the chromatographic conditions including column system,temperature program and modulation parameters,an analytical method of zedoary volatile oil was established based on comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry(GC×GC/TOFMS). The analysis of the individual components and groups were achieved. Under the optimized conditions the components of zedoary volatile oil from Guangxi province,China were identified by GC×GC/TOFMS. Among 249 components with similarities over 800,there are 18 terpenes,34 oxygenated terpenes,35 sesquiterpenes and 37 oxygenated sesquiterpenes. Sixty-nine of these components have the individual volume fractions of over 0.02%.

Key words: Volatile oil, Zedoary, Column system, Comprehensive two-dimensional gas chromatography, Time-of-flight mass spectrometry

中图分类号:

O655

TrendMD:

武建芳, 路鑫, 唐婉莹, 廉晓红, 孔宏伟, 阮春海, 许国旺. 全二维气相色谱/飞行时间质谱法在莪术挥发油组成分析中的应用. 高等学校化学学报, 2004, 25(8): 1432.

WU Jian-Fang, LU Xin, TANG Wan-Ying, LIAN Xiao-Hong, KONG Hong-Wei, RUAN Chun-Hai, XU Guo-Wang . Application of Comprehensive Two-dimensional Gas Chromatography/ Time-of-flight Mass Spectrometry to Analysis of Zedoary Volatile Oil. Chem. J. Chinese Universities, 2004, 25(8): 1432.

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