高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (7): 1396.doi: 10.7503/cjcu20140168

• 分析化学 • 上一篇    下一篇

葛花中异黄酮类化学成分的RRLC-Q-TOF MS/MS研究

戴雨霖1, 于珊珊1, 张颖1, 郝颖1, 钟薇1, 越皓1, 刘淑莹1,2()   

  1. 1. 长春中医药大学吉林省人参科学研究院, 长春 130117
    2. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2014-03-04 出版日期:2014-07-10 发布日期:2014-05-26
  • 作者简介:联系人简介: 刘淑莹, 女, 博士, 研究员, 博士生导师, 主要从事质谱分析研究. E-mail: syliu@ciac.jl.cn
  • 基金资助:
    国家自然科学基金(批准号: 21175127)资助

Studies on the Isoflavone in Extract of the Flower of Pueraria Lobata by RRLC-Q-TOF MS/MS

DAI Yulin1, YU Shanshan1, ZHANG Ying1, HAO Ying1, ZHONG Wei1, YUE Hao1, LIU Shuying1,2,*()   

  1. 1. Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
    2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2014-03-04 Online:2014-07-10 Published:2014-05-26
  • Contact: LIU Shuying E-mail:syliu@ciac.jl.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21175127)

摘要:

采用快速分离液相色谱-四极杆-飞行时间质谱联用(RRLC-Q-TOF MS/MS)技术对葛花中的异黄酮类成分进行了研究, 得到了相应化合物的保留时间、 分子量及结构信息. 研究结果表明, 葛花水提取液的主要成分为异黄酮类及其苷类化合物, 并含有皂苷类和酚类化合物. 在葛花中首次发现同属植物狐尾葛特有化合物Alopecuroides A和葛根中的化合物3'-甲氧基大豆苷元-7-O-甲醚. 讨论了异黄酮类化合物的质谱碎裂途径及规律.

关键词: 葛花, 异黄酮, 快速分离液相色谱-四极杆-飞行时间质谱

Abstract:

Rapid resolution liquid chromatography-electrospray ionization mass spectrometry(RRLC-ESI-MSn) was applied to identify and differentiate the isoflavone constituents of the flower of Pueraria lobata(Willd.)Ohwi. The data of retention time and molar masses were obtained for the identification of the major isoflavone constituents in the flower of Pueraria lobata(Willd.) Ohwi. The ESI-MSn data of the [M-H]- provide detailed structural information on the isoflavone. Alopecuroides A has been firstly detected from the Pueraria lobata(Willd.) Ohwi. flower. 28 components were detected in total from the flower of Pueraria lobata(Willd.) Ohwi..

Key words: Pueraria lobata(Willd.) Ohwi., Isoflavone, Rapid resolution liquid chromatography-electrospray ionization mass spectrometry(RRLC-ESI-MSn)

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