高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (10): 1866.

• 研究简报 • 上一篇    下一篇

反应时间、温度和溶剂对缬氨酸自组装成聚合肽反应的影响

李红1, 赵文杰1, 曹书霞2, 卢奎1, 赵玉芬2,3   

  1. 1. 河南工业大学化学化工系, 郑州450052;
    2. 郑州大学化学系, 化学生物学重点实验室, 郑州450052;
    3. 清华大学生命科学与工程研究院, 化学系, 生命有机磷化学及化学生物学教育部重点实验室, 北京100084
  • 收稿日期:2003-08-29 出版日期:2004-10-24 发布日期:2004-10-24
  • 基金资助:

    国家自然科学基金(批准号:20272055);河南省杰出青年科学基金(批准号:0312000900)资助

Influence of Reaction Time, Temperature and Solvent on the Self-assembly into Oligo-peptides for Valine

LI Hong1, ZHAO Wen-Jie1, CAO Shu-Xia2, LU Kui1, ZHAO Yu-Fen2,3   

  1. 1. Department of Chemistry and Chemical Engineering, Henan Industry University, Zhengzhou 450052, China;
    2. The Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China;
    3. The Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
  • Received:2003-08-29 Online:2004-10-24 Published:2004-10-24

关键词: 缬氨酸, 三氯氧磷, 寡肽, 电喷雾质谱

Abstract: Owing to having very strong bioactivity, oligo-peptides can be used as the medication or the pre-medication and become the studying focus now. Existing methods for synthesizing oligo-peptides included liquid phase peptide synthesis, solid phase peptide synthesis and enzymatic peptide synthesis. In this paper, a new method for synthesizing oligo-peptides by phosphorus oxychloride activation was reported. With the assistance of phosphorus oxychloride, the phosphorylation of L-valine could take place and then was assembled into oligo-peptides, which were analyzed by electrospray ionization mass spectrometry(ESI-MS). On quenching with methanol, the reaction mixtures, which were produced under different reaction conditions, yielded the corresponding peptide methyl esters. Moreover, we found that, as the reaction conditions such as reaction time, solvent and temperature were changed, the length of peptides changed. And the length of peptides could be controlled by changing the reaction conditions.

Key words: Valine, Phosphorus oxychloride, Oligo-peptide, Electrospray ionization mass spectrometry

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