复方中药四逆汤中乌头碱类二萜生物碱的电喷雾串联质谱研究

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (1): 85.

复方中药四逆汤中乌头碱类二萜生物碱的电喷雾串联质谱研究

王勇^1,2, 宋凤瑞¹, 金东明¹, 刘志强¹, 刘淑莹¹

1. 中国科学院长春应用化学研究所新药研究室, 长春 130022;
2. 东北师范大学化学学院, 长春 130024

收稿日期:2002-08-05 出版日期:2004-01-24 发布日期:2004-01-24
通讯作者: 刘淑莹(1942年出生),女,博士,研究员,博士生导师,从事质谱分析研究; 刘志强(1962年出生),男,博士,研究员,博士生导师,从事质谱分析研究.E-mail:mslab@ciac.jl.cn E-mail:mslab@ciac.jl.cn
基金资助:
国家自然科学基金(批准号:30271600)资助

Studies on the Aconitum Alkaloids in the Sini Concoction by Electrospray Ionization Mass Spectrometry

WANG Yong^1,2, SONG Feng-Rui¹, JIN Dong-Ming¹, LIU Zhi-Qiang¹, LIU Shu-Ying¹

1. The New Drug Lab of Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Faculty of Chemistry, Northeast Normal University, Changchun 130024, China

Received:2002-08-05 Online:2004-01-24 Published:2004-01-24

摘要/Abstract

摘要： 利用电喷雾串联质谱方法不经柱分离而直接分析复方中药四逆汤中二萜生物碱的组成,乌头碱类二萜生物碱在质谱条件下形成质子化分子,一种生物碱对应一个分子离子峰.检测到了苯甲酰单酯型、双酯型和脂类生物碱等18种二萜生物碱,其中苯甲酰中乌头原碱等单酯型生物碱是四逆汤中的主要生物碱成分.

关键词: 电喷雾质谱, 四逆汤, 乌头碱, 单酯型生物碱

Abstract: Electrospray ionization mass spectrometry(ESI-MS) was used for the analysis of the aconitum alkaloids in the complex prescription of TCM, Sini concoction, which consists of prepared aconite roots, liquorice and dried ginger, without needing chromatographic separation. [M⁺H]+ are formed for aconitum alkaloids in the positive ion mode. Eighteen aconitum alkaloids including benzoylaconines, aconitines and lipo-alkaloids were found. Benzoylaconines were detected as the main aconitum alkaloids in Sini concoction.

Key words: Electrospray ionization mass spectrometry, Sini concoction, Aconitine, Mono-ester aconitum alkaloids

中图分类号:

O657

TrendMD:

王勇, 宋凤瑞, 金东明, 刘志强, 刘淑莹. 复方中药四逆汤中乌头碱类二萜生物碱的电喷雾串联质谱研究. 高等学校化学学报, 2004, 25(1): 85.

WANG Yong, SONG Feng-Rui, JIN Dong-Ming, LIU Zhi-Qiang, LIU Shu-Ying . Studies on the Aconitum Alkaloids in the Sini Concoction by Electrospray Ionization Mass Spectrometry. Chem. J. Chinese Universities, 2004, 25(1): 85.

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