高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (7): 1207.

• 研究简报 • 上一篇    下一篇

液相色谱Z值对溶菌酶分子构象变化的定量表征

王彦, 李敏, 龚波林, 耿信笃   

  1. 西北大学现代分离研究所, 分离科学陕西省重点实验室, 西安 710069
  • 收稿日期:2002-07-12 出版日期:2003-07-24 发布日期:2003-07-24
  • 通讯作者: 耿信笃(1941年出生),男,教授,博士生导师,主要从事分离科学研究.E-mail:xdgeng@nwu.edu.cn E-mail:xdgeng@nwu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20175016)资助

Quantitative Characterization of Molecular Conformation of Lysozyme with Z in Liquid Chromatography

WANG Yan, LI Min, GONG Bo-Lin, GENG Xin-Du   

  1. Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi′an 710069, China
  • Received:2002-07-12 Online:2003-07-24 Published:2003-07-24

关键词: 溶菌酶, 液相色谱, 变性, 构象, 表征

Abstract:

Different molecular conformations, i.e., native, urea-unfolded, guanidine hydrochloride(GuHCl)-unfolded, urea-reduced-unfolded, GuHCl-reduced-unfolded, of lysozyme was systematically studied by reversed-phase liquid chromatography(RPLC), and weak-cation exchange chromatography(WCX), and the stoichiometric parameters Z in stoichiometeric displacement model(SDM). The difference of molecular conformations of lysozyme with their disulfide bonds cleaved or could not be found in RPLC and WCX in terms of retention. However, they could not be used to distinguish their reduced states from non-reduced ones. Each difference of molecular conformations of lysozyme, caused by urea and GuHCl solution, can only be characterized with Z. The definition of the degree of the unfolded was introduced to characterize the change of conformation.

Key words: Lysozyme, Liquid chromatography, Unfolded, Conformation, Characterization

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