高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (5): 935.

• 论文 • 上一篇    下一篇

聚偏氟乙烯中空纤维亲和膜分离γ-球蛋白的研究(Ⅰ)--聚偏氟乙烯中空纤维亲和膜的制备及其吸附性能

虞骥, 甘宏宇, 何奕, 袁骏, 陈欢林   

  1. 浙江大学材料与化工学院, 杭州 310027
  • 收稿日期:2002-02-06 出版日期:2003-05-24 发布日期:2003-05-24
  • 通讯作者: 陈欢林(1949年出生),男,教授,博士生导师,主要从事膜与膜技术研究.E-mail:chenhl@che.zju.edu.cn E-mail:chenhl@che.zju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29676038);中国博士后科学基金会科研基金

Studies on PVDF Hollow Fiber Affinity Membrane for Separation of γ-Globulin from Human Plasma(Ⅰ) -Preparation of PVDF Hollow Fiber Affinity Membrane and Its Adsorption Properties

YU Ji, CAN Hong-Yu, HE Yi, YUAN Juan, CHEN Huan-Lin   

  1. College of Materials Science and Chemistry Engineering, ZheJiang University, Hangzhou 310027, China
  • Received:2002-02-06 Online:2003-05-24 Published:2003-05-24

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摘要： 对聚偏氟乙烯(PVDF)中空纤维膜进行表面亲水化处理,再经活化、偶联制得了带有苯丙氨酸(Phe)的PVDF中空纤维亲和膜.制备过程中以光电子能谱(XPS)及红外光谱分析膜表面基团的变化,经亲水化处理及活化偶联后,膜表面的O和N含量分别从5.6％和0.6％增加到16.5％和3.0％,而F含量由38.3％降低为23.0％,将该亲和膜用于室温下血浆中γ-球蛋白的分离实验,在pH=7.4,离子强度I=0.18的缓冲液体系及进料质量浓度为1.0mg/mL条件下,该亲和膜对γ-球蛋白的吸附量可达到5.85mg/g膜;与市售γ-球蛋白相比,血浆中提取纯度可达83.9％.

关键词: 聚偏氟乙烯, 中空纤维膜, 亲和吸附, XPS分析, &gamma, -球蛋白

Abstract: Poly(vinylidene fluoride) (PVDF) hollow fiber membrane used as the matrix was hydrophile-modified by alkali and hydroxyethyl-cellulose(HEC) treatment in this paper. After being activated and coupled, the PVDF hollow fiber affinity membranes with phenylalanine as the ligand were prepared. XPS analysis was used for measuring the changes of chemical structure on the membrane surface. The results indicated that on the membrane surface oxygen and nitrogen contents increased from 5. 6% to 16. 5% and 0. 6% to 3. 0% respectively, while fluorine content decreased from 38. 3% to 23. 0%. The affinity membrane was used for separating γ-globulin (HGG) from human plasma. The result showed that adsorption capacity of HGGby the affinity membrane was 5. 85 mg/g membrane under the condition of pH=7. 4, I=0. 18(ionic strength), ρ=1. 0 mg/mL(feed liquid) and the purity of HGGextracted from human plasma was up to 83. 9%.

Key words: Poly(vinylidene fluoride)(PVDF), Hollow fiber membrane, Affinity adsorption, XPS analy-sis, γ-Globulin

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