基于可控定向PVDF电纺纤维构建细胞相容性的三维微环境

doi:10.7503/cjcu20160832

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (6): 1002.doi: 10.7503/cjcu20160832

• 中国第四届静电纺丝大会专题研究论文 • 上一篇下一篇

基于可控定向PVDF电纺纤维构建细胞相容性的三维微环境

靳林^1,²(), 徐钦炜³, 胡彬¹, 黄敬斌¹, 张宜磊³, 王振领^1,²()

1. 周口师范学院稀土功能材料及应用重点实验室, 周口 466001
2. 河南省生物医用纳米材料国际联合实验室, 周口 466001
3. 新加坡南洋理工大学机械与宇航学院, 新加坡 639798

收稿日期:2016-11-25 出版日期:2017-06-10 发布日期:2017-05-18
作者简介:联系人简介: 靳林, 博士, 副教授, 主要从事纳米纤维的制备及应用. E-mail: jinlin_1982@126.com; 王振领, 博士, 教授, 主要从事纳米发光材料的制备及应用. E-mail: zlwang2007@hotmail.com
基金资助:
国家自然科学基金 (批准号: 21404124, 51572303)、河南省高校科技创新人才(批准号: 17HASTIT007)、河南省创新型科技人才队伍建设工程(批准号: 2013259)、河南省应用化学重点学科(批准号: 201218692)、河南省高校创新团队(批准号: 14IRTSTHN009)和河南省科技计划项目(批准号: 162102310591)资助.

Tuneable Three-dimensional Cytocompatible Micro-environments Designed by Controlled Alignment of PVDF Electrospun Fibres

JIN Lin^1,^2,^*(), XU Qinwei³, HU Bin¹, HUANG Jingbin¹, ZHANG Yilei³, WANG Zhenling^1,^2,^*()

1. Key Laboratory of Rare Earth Functional Materials and Applications,Zhoukou Normal University, Zhoukou 466001, China
2. International Joint Research Laboratory for Biomedical Nanomaterials of Henan, Zhoukou 466001, China
3. School of Mechanical & Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore;

Received:2016-11-25 Online:2017-06-10 Published:2017-05-18
Contact: JIN Lin,WANG Zhenling E-mail:jinlin_1982@126.com;zlwang2007@hotmail.com
Supported by:
This paper is supported by the National Natural Science Foundation of China(Nos.21404124, 51572303), the Program of Innovative Talent(in Science and Technology) in University of Henan Province, China(No.17HASTIT007), the Project of Innovation Scientists and Technicians Troop Construction Projects of Henan Province, China(No.2013259), the Project of Henan Province Key Discipline of Applied Chemistry, China(No.201218692), the Program for Innovative Research Team(in Science and Technology) in University of Henan Province, China(No.14IRTSTHN009) and the Project of Science and Technology Program of Henan Province, China(No.162102310591).

摘要/Abstract

摘要：

三维电纺纤维在生物医学领域, 如生物传感、药物控制释放与组织工程等方面具有良好的应用前景. 然而, 现有的电纺技术在制备结构、孔隙率与形貌均可调节的三维定向电纺纤维方面还存在一定不足. 因此亟需开发一种新型的电纺丝工艺以制备三维定向电纺纤维. 本文通过改进传统的电纺丝工艺, 开发了一种简单高效制备三维定向聚偏氟乙烯(PVDF)的电纺丝制备技术. 所制备的三维定向纤维的形貌、直径及纤维密度均可控. 体外细胞实验结果表明, 该类三维定向纤维具有良好的生物相容性, 能够促进细胞活性, 诱导细胞沿着纤维的方向生长. 此外, 研究结果还表明, 将该三维定向纤维作为细胞培养支架时, 细胞的增殖高于利用传统的二维纤维膜. 该制备技术将极大地拓宽三维定向纤维在三维细胞培养、组织工程及疾病诊断等生物医学领域的应用.

关键词: 静电纺丝, 聚偏氟乙烯, 骨髓间充质干细胞, 组织工程

Abstract:

Three dimensional(3D) aligned electrospun fibres have a promising potential application in biomedical areas, such as biosensors, controlled drug release and tissue engineering. However, the fabrication of these fibres with tuneable microarchitectures, porosity and overall morphology still has challenges due to electrospinning process. Thus, it is highly desired to develop novel, cost-effective and easily scalable fabrication methods for the 3D aligned electrospun fibres. Herein, we developed a facile yet effective method for the preparation of 3D poly(vinylidene fluoride)(PVDF) aligned fibres(3D AFs) via an improved electrospinning technique. The obtained 3D AFs showed controllable morphology, diameter and fiber density. Furthermore, the obtained 3D AFs showed excellent in vitro biocompatibility. Moreover, the as-prepared 3D AFs enhanced cellular activities and induced directional cell growth along the direction of nanofibre orientation, thereby providing an excellent cue for the anchorage and migration of human mesenchymal stem cells(hMSCs). More importantly, cell proliferation in the 3D AFs was found to be significantly higher than that on the nanofibre mats(NFMs). Combined with controllable morphology and structure, we anticipate that this finding greatly enhances the potential applications of these 3D AFs for therapeutically relevant 3D cell cultures, tissue engineering, diagnostics and other biomedical applications.

Key words: Electrospinning, Poly(vinylidene fluoride)(PVDF), Human mesenchymal stem cell(hMSC), Tissue engingeering

中图分类号:

O632.21

TrendMD:

靳林, 徐钦炜, 胡彬, 黄敬斌, 张宜磊, 王振领. 基于可控定向PVDF电纺纤维构建细胞相容性的三维微环境. 高等学校化学学报, 2017, 38(6): 1002.

JIN Lin, XU Qinwei, HU Bin, HUANG Jingbin, ZHANG Yilei, WANG Zhenling. Tuneable Three-dimensional Cytocompatible Micro-environments Designed by Controlled Alignment of PVDF Electrospun Fibres. Chem. J. Chinese Universities, 2017, 38(6): 1002.

图/表 7

Fig.1 Illustration of the improved electrospinning process with the special co-axial frisbee disc collectors arranged on a rotary shaft for the fabrication of 3D aligned nanofibres(A), photographic image of the aligned 3D oriented nanofibres(B), SEM images of a traditional PVDF aligned fibre mesh(2D AFs, C) and 3D fluffy PVDF aligned fibres(3D AFs, D)

Fig.2 BET surface area of 2D random nanofibres, 2D aligned nanofibres and 3D aligned nanofibres

Fig.3 SEM images of 3D aligned nanofibres showing differences in nanofiber diameters(A1—C1) and the nanofiber diameter distribution within the scaffolds(A2—C2) with respect to PVDF concentrationPVDF concentration(%): (A) 13; (B) 16; (C) 18.

Fig.4 SEM images of 3D aligned nanofibres with different densitiesCollecting time/min: (A) 30; (B) 60.

Fig.5 SEM images(day 5) of hMSCs cultured on the 2D random nanofibres(A), 2D aligned nanofibres(B) and 3D aligned nanofibres(C)

Fig.6 Fluorescence images of hMSCs cultured for 5 d on/in 2D random nanofibres(A), 2D aligned nanofibres(B) and 3D aligned nanofibres(C)

Fig.7 Proliferation of hMSCs cultured for various periods on/in 2D random nanofibres, 2D aligned nanofibres and 3D aligned nanofibres

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基于可控定向PVDF电纺纤维构建细胞相容性的三维微环境

Tuneable Three-dimensional Cytocompatible Micro-environments Designed by Controlled Alignment of PVDF Electrospun Fibres

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