高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1081.

• 研究论文 • 上一篇    下一篇

转基因水稻表达的Bt毒蛋白CryⅠ Ab溶液构象的研究

吴志平1,2, 舒庆尧2, 龚利琴2, 余红3, 叶恭银4, 陈子元4   

  1. 1. 浙江大学化学系, 杭州 310029;
    2. 浙江大学原子核农业科学研究所, 杭州 310029;
    3. 浙江大学生物系, 杭州 310029;
    4. 浙江大学植保系, 杭州 310029
  • 收稿日期:2001-03-12 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 吴志平(1965年出生),男,博士,副教授,主要从事蛋白质生物化学与分子生物学方面的研究.E-mail:wzpzju@263.net E-mail:wzpzju@263.net
  • 基金资助:

    浙江省院士基金;教委科技发展基金资助

Studies on the Solution Conformations of Bt Toxic CryⅠ Ab Expressed by Bt Transgenic Rice

WU Zhi-Ping1,2, XU Bu-Jin2, SHU Qing-Yao2, GONG Li-Qin3, YU Hong4, YE Gong-Yin4, CHEN Zi-Yuan2   

  1. 1. Department of Chemistry, Hangzhou 310029, China;
    2. Institute of Nuclear Agricultural Sciences, Hangzhou 310029, China;
    3. Department of Biology, Hangzhou 310029, China;
    4. Department of Plan Protection, Zhejiang University, Hangzhou 310029, China
  • Received:2001-03-12 Online:2002-06-24 Published:2002-06-24

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被引次数

2

摘要： 用荧光光谱法研究了毒蛋白CryAb在溶液中的构象.结果表明,pH值增大时,毒蛋白的荧光强度也增大,至pH=11时荧光强度已达最大值;甲醇、乙醇及异丙醇等有机溶剂引起毒蛋白荧光发射波长蓝移和荧光强度增大,至有机溶剂的浓度超过某一阈值后荧光发射波长不再蓝移;丙酮能完全猝灭毒蛋白CryAb的荧光,丙烯酰胺能猝灭CryAb 86%的荧光,而KI对毒蛋白的荧光无猝灭作用,由此推断CryAb的大部分色氨酸残基位于分子表面富含负电荷的区域中.

关键词: 转基因水稻, Bt毒蛋白CryⅠ Ab, 构象

Abstract: The conformations of the toxic protein CryⅠAb in its solution were studied by fluorescence. The results showed that the toxic protein fluorescence intensity is improved with its pHvalue increase and reaches its maximum at pHequivalent to 11. Adding some organic solvents such as methanol, ethanol, isopropanol result in the emission wavelength of fluorescence of the toxic protein blue shift and its fluorescence intensity increase. However, the toxic protein fluorescence emission wavelength no long blue shifts as these organic solvents concentration beyond certain values. Acetone can quench all fluorescence of the toxic protein CryⅠAb and acrylamide can quench 86% of the total fluorescence of the toxic protein, but KIcan′t quench any fluorescence. It implicates that the most of tryptophan residuals of CryⅠAb are located on the toxic protein surface where the negative charge is rich.

Key words: Transgenic rice, Bt toxic protein CryⅠAb, Conformation

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