高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1044.

• 研究简报 • 上一篇    下一篇

压电免疫传感器用于乙肝表面抗原的测定

陈泽忠1, 王柯敏1, 陈永康2, 肖丹1, 羊小海1, 邵国强2   

  1. 1. 化学生物传感与计量学国家重点实验室, 湖南大学化学化工学院, 长沙 410082;
    2. 香港浸会大学化学系, 香港
  • 收稿日期:2001-03-06 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 王柯敏(1957年出生),男,教授,博士生导师,从事化学传感器及纳米尺度上获取生物化学信息的研究.
  • 基金资助:

    TheResearchGrantsCounciloftheHongKongSpecialAdministrationRegion;China(No.HKBU2054/98p);国家杰出青年基金(批准号:29825110);湖南省自然科学基金(批准号:00GKY1010)资助

Development of a Piezoelectric Immunosensor for Hepatitis B Surface Antigen

CHEN Ze-Zhong1, WANG Ke-Min1, CHEN Yong-Kang2, XIAOD an1, YANG Xiao-Hai1, SHAO Guo-Qiang2   

  1. 1. State Key Lab of Chem biological Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
    2. Department of Chemistry, B aptist University, Hong Kong, China
  • Received:2001-03-06 Online:2002-06-24 Published:2002-06-24

PDF (PC)

被引次数

39

关键词: 压电免疫传感器, 乙肝表面抗原, 自组装膜

Abstract: Apiezoelectric immunosensor has been developed for the detection of Hepatitis Bsurface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self-assemble monolayers(SAMs). Using a bifunctional cross-linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0.2-1.20 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0.2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.

Key words: Piezoelectric immunosensor, Hepatieis B surface antigen, Self-assemble monolayers

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