高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (5): 852.

• 研究简报 • 上一篇    下一篇

磷酸化血管紧张素Ⅱ的合成及性质

罗施中1, 赵刚1, 李艳梅1, 赵玉芬2, 许莉1   

  1. 1. 清华大学化学系, 生命有机磷化学教育部重点实验室, 北京 100084;
    2. 北京微量化学研究所, 北京 100091
  • 收稿日期:2001-01-07 出版日期:2002-05-24 发布日期:2002-05-24
  • 通讯作者: 李艳梅(1964年出生),女,博士,教授,博士生导师,从事生物有机化学研究.E-mail:liym@mail.tsinghua.edu.cn E-mail:liym@mail.tsinghua.edu.cn
  • 基金资助:

    教育部高等学校优秀青年教师教学和科研奖励基金;国家自然科学基金(批准号:20072023)资助.

Synthesis and Characterization of Phosphoangiotensin Ⅱ

LUO Shi-Zhong1, ZHAO Gang1, LI Yan-Mei1, XU Li2, ZHAO Yu-Fen1   

  1. 1. Key Laboratory for Biaorganic Phosphorus Chemistry of Education Ministry, Department of Chemistry, Tsinghua University, Beijing 100084, China;
    2. Beijing Institute of Microchemistry, Beijing 100091, China
  • Received:2001-01-07 Online:2002-05-24 Published:2002-05-24

关键词: 磷酸化血管紧张素Ⅱ, 总体磷酸化法, 磷酸化单体法, 电喷雾质谱, CD光谱

Abstract: The reversible phosphorylation/dephosphorylation of protein is probably the most common andimportant regulatory modification of proteins.Synthesis and identification of phosphopeptide have beenshown to have dramatic agonist effects in several signaling pathways.The phosphoangiotensin Iwas syn-thesized with two different strategies: global approach and building block approach.The building block ap-proach using phosphorylation regeants di-tert-butyl-N,N-diethyl phosphoramidite is the preferred methodfor the synthesis of peptides containing phosphotyrosine.The phosphoangiotensin and angiotensin were easily distinguished by ESI-MS.It may be useful to determining the phosphrylation sites of peptidescontaining multi-phosphorylated tyrosines by multi-stage mass.The phosphorylation of angiotensin Ide-creases the content of-helix and increases the contents of -turn.

Key words: Phosphoangiotensin Ⅱ, Global approach, Building block approach, ESI-MS, CD spectra

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