高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (4): 564.

• 研究快报 • 上一篇    下一篇

加替沙星铽敏化化学发光与应用

连宁1, 赵慧春1, 孙春燕1, 金林培1, 张仲伦2, 郑雁珍2   

  1. 1. 北京师范大学化学系, 北京 100875;
    2. 中国科学院生物物理研究所, 北京 100101
  • 收稿日期:2001-11-20 出版日期:2002-04-24 发布日期:2002-04-24
  • 通讯作者: 赵慧春(1946年出生),女,教授,主要从事光谱分析化学研究.E-mail:zhaohuichun@263.net E-mail:zhaohuichun@263.net
  • 基金资助:

    国家自然科学基金(批准号:29971005);国家重点基础研究发展规划项目(批准号:G1998061308)及北京市自然科学基金资助.

Terbium Sensitized Chemiluminescence of Gatifloxacin and Its Application

LIAN Ning1, ZHAO Hui-Chun1, SUN Chun-Yan1, JIN Lin-Pei1, ZHANG Zhong-Lun2, ZHENG Yan-Zhen2   

  1. 1. Department of Chemistry, Beijing Normal University, Beijing 100875, China;
    2. Institute of Biology and Physics, Chinese Academy of Sciences, Beijing 100084, China
  • Received:2001-11-20 Online:2002-04-24 Published:2002-04-24

PDF (PC)

被引次数

66

关键词: 加替沙星, 铽, 化学发光, 能量转移, 流动注射

Abstract: A selective and sensitbe sensitized chemiluminescence (CL) method for the determination ofGatifloxacin(GFLX) is described.The method is based on Tb3+ sensitized chemiluminescence of the Tb3+-GFLX-Ce(Ⅳ)-SO32- system since CLfor the binary Ce(Ⅳ)-SO32- system is weak, while CL is enhanced when Tb3+-GFLX is added to the Ce(Ⅳ)-SO32- system.The possible mechanism of the CLreaction maybe that Ce(Ⅳ) can oxidize HSO3- to a hydrogen-sulfite radical HSO3, then HSO3 radicals produce S2O62-,S2O62- will form the excited intermediate product SO2. The energy of SO2 is mainly transferred to Tb3+-GFLX, and the CLspectrum shows a characteristic emission of Tb3+.The re1ationship between the CLin-tensity and the concentration of GFLXis linear in the range of 2.0 × 10-8-1.0×10-6 mol/L, and the de-tection limit is 3.5 × 10-9 mol/L.The relative standard deviation(RSD) for 11 independent determinationsof 1.0×10-7 mol/L GFLX was 3.2%.Common excipient starch was used in pharmaceutical preparationand most of metal ions do not interfere with the determination of GFLX.GFLXin urine can be analyzeddirectly after appropriate dilution of the samples, and the analytical result is satisfactory.

Key words: Gatifloxacin, Terbium, Chemiluminescence, Energy transfer, Flow injection

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