高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (3): 408-415.doi: 10.7503/cjcu20170667

• 研究论文: 无机化学 • 上一篇    下一篇

荧光铁载体-铽(Ⅲ)-环丙沙星三元配合物的光谱研究

宋凯丽, 杨斌盛()   

  1. 山西大学分子科学研究所, 化学生物学与分子工程教育部重点实验室, 太原 030006
  • 收稿日期:2017-10-09 出版日期:2018-03-10 发布日期:2018-01-13
  • 作者简介:联系人简介: 杨斌盛, 男, 教授, 博士生导师, 主要从事生物无机化学研究. E-mail:yangbs@sxu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21571117)资助

Spectroscopic Study on Ternary Complexes of Pyoverdine-terbium(Ⅲ)-ciprofloxacin

SONG Kaili, YANG Binsheng*()   

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
  • Received:2017-10-09 Online:2018-03-10 Published:2018-01-13
  • Contact: YANG Binsheng E-mail:yangbs@sxu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21571117)

摘要:

在pH=8.0的50 mmol/L Tris-HCl缓冲溶液中, 利用紫外光谱、 荧光光谱、 荧光寿命和等温滴定量热分析研究了环丙沙星(CIP)、 Tb3+与荧光铁载体(Pvd)三者之间的结合性质. 结果表明, CIP与Tb3+以3∶1摩尔比结合, 结合常数约为1013 L3/mol3, Tb(CIP)3配合物的形成使Tb3+在544 nm处的荧光敏化. Pvd可与Tb3+以1∶1摩尔比结合, Pvd-Tb3+配合物在544 nm处并未出现Tb3+的荧光敏化峰. 三者之间的荧光滴定实验与等温滴定量热分析均证明了Pvd-Tb3+-CIP三元配合物的形成, 其中CIP与Pvd-Tb3+的结合常数为(3.10±0.39)×103 L/mol. 配合物中CIP对Tb3+特征荧光的敏化效应为抗菌素抗荧光假单胞菌机理的深入研究提供了可行的方法.

关键词: 荧光铁载体, 环丙沙星, 铽(Ⅲ), 荧光敏化

Abstract:

The binding properties of ciprofloxacin(CIP), terbium and pyoverdine(Pvd) were studied by UV-Vis spectroscopy, fluorescence spectroscopy, fluorescence lifetime measurement and isothermal titration calorimetry(ITC) in pH=8.0, 50 mmol/L Tris-HCl buffer. The results show that the characteristic sensitization peak of terbium is generated at 544 nm upon CIP, forming a 3∶1 complex with terbium, the binding constant is approach to 1013 L3/mol3. Pvd binds to terbium at 1∶1, representing no characteristic sensitization peak at 544 nm. More importantly, it is founded that Pvd-Tb3+-CIP ternary complex forms based on the different fluorescence sensitization properties of Pvd and CIP on terbium. The ITC data reveal that the binding constant for the binding of CIP to Pvd-Tb3+ is (3.10±0.39)×103 L/mol. Consequently, fluorescence lifetime of CIP changes much as a result of the formation of ternary complexes. The characteristic fluorescence sensitization effect of CIP to Tb3+ provides a feasible method for the further study of the antibiotic resistance of pseudomonas aeruginosa.

Key words: Pyoverdine, Ciprofloxacin, Terbium, Fluorescence sensitization

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