高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (9): 1462.

• 研究论文 • 上一篇    下一篇

鲨鱼软骨血管形成抑制因子的分离纯化及其活性的初步研究

曾锋1, 谢团霞1, 杨伟兴1, 张展霞1, 余健秀2, 庞义2   

  1. 1. 中山大学化学与化学工程学院, 广州 510275;
    2. 中山大学生命科学学院, 广州 510275
  • 收稿日期:2000-10-26 出版日期:2001-09-24 发布日期:2001-09-24
  • 通讯作者: 曾　锋(1964年出生),男,博士,副教授,从事生物分析化学及环境微生物学等方面的研究.
  • 基金资助:

    广州市科学技术委员会科技攻关项目(批准号:98-J-002-01)资助

A Preliminary Study of Purification and Bioactivity of a 12600 Shark Cartilage Angiogenesis Inhibitor

ZENG Feng1, XIE Tuan-Xia1, YANG Wei-Xing1, ZHANG Zhan-Xia1, YU Jian-Xiu2, PANG Yi2   

  1. 1. School of Chemistryand Chemical Engineering, Zhongshan University;
    2. School of Life Sciences, Zhongshan University, Guangzhou 510275, China
  • Received:2000-10-26 Online:2001-09-24 Published:2001-09-24

PDF (PC)

被引次数

11

摘要： 以广东阳江鲨鱼软骨为原料,研究了鲨鱼软骨新生血管形成抑制因子的分离纯化方法,并对其生物学活性进行了初步研究.采用盐酸胍抽提、膜超滤、丙酮分级沉淀、SephadexG-75柱层析和C4反相高效液相色谱等步骤,分离纯化出一种新的鲨鱼软骨血管生成抑制因子-a(SharkCartilageAngiogenesisInhibitor-a,SCAI-a),分子量为12600,能显著抑制鸡胚绒毛尿囊膜血管的形成.

关键词: 鲨鱼软骨, 蛋白质, 分离纯化, 生物活性

Abstract: The purification method and bioactivity of shark cartilage angiogenesis inhibitor a(SCAIa) from Guangdong Yangjiang were investigated. The 1.0 mol/Lguanidine and 002 mol/L MES(at 28 ℃ ) extracts of shark cartilage was centrifuged at 6000 r/min for 30 min and the pellet was discarded, and the supernatant was recentrifuged at 12000 r/min for 30 min, then ultrafiltrated and fractionally precipitated with 25%-90% acetone. The precipitate was applied to a Sephadex G 75 gel column and eluted after the predominant peak of A280nm absorbing material with 0.05 mol/L Tris HCl buffer(containing 0.02mol/L NaCl and 0.02% NaN3). The material Bwere composed of three single peptides with molecular mass of 18000, 15000 and 12600, then were purified using reverse phase high performance liquid chromatography, the eluate was collected, dried and observed by chichen chorioallantoic membrane. The pure inhibitor was homogeneous as a single band on a silver stained 125% sodium dodecyl sulfate polyacrylamide gel. SCAIa had a molecular weight of 12600. It specifically inhibited the chorioallantoic membrane of chick embryos.

Key words: Shark cartilage, Protein, Purification, Bioactivity

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