高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (1): 21.

• 研究论文 • 上一篇    下一篇

La(Ⅲ)与HSA或BSA的结合平衡研究

梁宏1, 边贺东1, 涂楚桥1, 申泮文2   

  1. 1. 广西师范大学生物无机化学研究所, 化学化工系, 桂林541004;
    2. 南开大学化学系, 天津300071
  • 收稿日期:1999-12-21 出版日期:2001-01-24 发布日期:2001-01-24
  • 通讯作者: 梁　宏(1964年出生),男,博士,教授,从事生物无机化学和配位化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29961001);广西十百千人才工程基金;广西高校自然科学基金

Binding Equilibrium Study Between La(Ⅲ) and HSA or BSA

LIANG Hong1, BIAN He-Dong1, TU Chu-Qiao1, SHEN Pan-Wen2   

  1. 1. Research Institute of Bioinorganic Chemistry, Department of Chemistryand Chemical Engineering, Guangxi Normal University, Guilin 541004, China;
    2. Department of Chemistry, Nankai University, Tianjin 300071, China
  • Received:1999-12-21 Online:2001-01-24 Published:2001-01-24

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被引次数

52

摘要： 用平衡透析法详细研究了pH=6.3条件下La(Ⅲ)与HSA或BSA的结合平衡.Scatchard图分析表明,La(Ⅲ)在HSA中有2个强结合部位和8个弱结合部位;在BSA中有2个强结合部位和6个弱结合部位.从La(Ⅲ)与Cu(Ⅱ),Zn(Ⅱ)和Cd(Ⅱ)等的竞争结合HSA或BSA的结果推测:La(Ⅲ)在HSA或BSA中的一个强结合部位的配位原子可能全部是氧原子.通过非线性最小二乘法拟合Bjerrum方程,首次报道了La(Ⅲ)-HSA和La(Ⅲ)-BSA体系的逐级稳定常数值,其K1的数量级为104.Hill系数及自由能偶合分析表明La(Ⅲ)与HSA或BSA的结合均产生一定的负协同效应.

关键词: La(Ⅲ)-血清白蛋白, 平衡透析, 配位分析, 逐级稳定常数, 协同效应

Abstract: The binding of La(Ⅲ) to human serum albumin (HSA) or bovine serum albumin(BSA) has been studied by equilibrium dialysis at pH6.3. The results from Scatchard plots indicate that there are two strong sites and eight weak sites in HSA and two strong sites and six weak sites in BSA, respectively. The results of La(Ⅲ)competing with Cu(Ⅱ), Zn(Ⅱ), Cd(Ⅱ) for binding to HSAor BS Asuggest that one of the strong binding sites is most probably coordinated with atoms which are almost all oxygen. The successive stability constants which are reported for the first time are obtained by non-linear least square method fitting Bjerrum formula. For both La(Ⅲ)-HSA and La(Ⅲ)-BSA systems, the order of magnitude of K1 was found to be 104. The analyses of Hill coefficient and free energy coupling show that some negative cooperative effect was found in both La(Ⅲ)-HSA and La(Ⅲ)-BSA systems.

Key words: La(Ⅲ)-serum albumin, Equilibrium dialysis, Coordination analysis, Successive stability constant, Cooperative effect

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