高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (6): 876.

• 研究简报 • 上一篇    下一篇

苯二酚异构体的高效液相色谱化学发光检测法研究

崔华, 贺彩霞, 赵贵文   

  1. 中国科学技术大学化学系, 合肥 230026
  • 收稿日期:1999-10-02 出版日期:2000-06-24 发布日期:2000-06-24
  • 通讯作者: 崔 华(1963年出生),女,博士,副教授,主要从事化学发光分析研究.3现在华东理工大学分析测试中心工作.
  • 基金资助:

    中国科技大学青年科学基金

The Determination of Isomers of Dihydroxybenzenes by High Performance Liquid Chromatography with ChemiluminescenTdetection

CUI Hua, HE Cai-Xia, ZHAO Gui-Wen   

  1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
  • Received:1999-10-02 Online:2000-06-24 Published:2000-06-24

关键词: 化学发光, 高效液相色谱, 对苯二酚, 邻苯二酚, 间苯二酚

Abstract: Based on the inhibition by hydroquinone, catechol and resorcinol of chemiluminescence from luminol potassium hexacyanoferrate(Ⅲ) system, a high performance liquid chromatographic procedure with chemiluminescenTdetection was developed for simultaneous determination of hydroquinone, catechol and resorcinol. The separation was carried out on RP-C18 column at 37 ℃ by using a step gradient elution made by the mobile phases of methanol(A) and 0.05% H3PO4(B). The gradient elution condition was 95% B, 5 min; 95%B-50%B, 1 min; 50% B, 6 min. This method has the merits of high senstivity and good selectivity. The detection limits for hydroquinone, catechol and resorcinol are 2.0×10-9 , 3.2×10-9 and 6.8×10-7 g/mL, respectively. The relative standard deviation( n =5) is 1.9% for 2.5×10-7 g/mLhydroquinone, 1.0% for 7.5×10-7 g/mLcatechol, 4.0% for 5.0×10-5 g/mLresorcinol, respectively. The recovery is in the range of 94%—104%.

Key words: Chemiluminescence, High performance liquid chromatography, Hydroquinone, Catechol, Resorcinol

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