高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (7): 1106.

• 论文 • 上一篇    下一篇

螯合剂对铽(Ⅲ)-转铁蛋白配合物的影响

张爱萍, 杨斌盛   

  1. 山西大学分子科学研究所, 太原 030006
  • 收稿日期:1998-09-04 出版日期:1999-07-24 发布日期:1999-07-24
  • 通讯作者: 杨斌盛
  • 作者简介:张爱萍,女,33岁,硕士,讲师,现在山西医科大学基础部工作.
  • 基金资助:

    山西省留学归国人员基金

Effect of Chelate Reagents on Terbium-apotransferrin

ZHANG Ai-Ping, YANG Bin-Sheng   

  1. Institute of Molecular Science, Shanxi University, Taiyuan, 030006
  • Received:1998-09-04 Online:1999-07-24 Published:1999-07-24

PDF (PC)

被引次数

17

摘要： 在室温及pH7.4下,观察了一些螯合剂对铽(Ⅲ)-转铁蛋白配合物的影响.结果表明,铽(Ⅲ)与人血清脱铁转铁蛋白的结合是可逆的;EDTA和次氮基三乙酸可使铽(Ⅲ)-脱铁转铁蛋白配合物定量释放铽(Ⅲ).用ECCLES程序计算表明,血清中微摩尔数量级的铽(Ⅲ)主要以小分子配合物形式存在,其中铽(Ⅲ)-柠檬酸约占62%,而铽(Ⅲ)-脱铁转铁蛋白配合物约占21%.

关键词: 转铁蛋白, 螯合剂, 物种, 铽(Ⅲ)

Abstract: The effect of chelate reagents on Tb3+-transferrin was studied at room temperature and pH7.4. The results show that the binding of Tb3+to apotransferrin is reversible in 0.1 mol/L N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid and pH7.4. Ethylenediamine tetraacetic acid, and nitrilotriacetic acid can make Tb3+-transferrin dissociated completely. The species of Tb3+in serum was calculated by using ECCLES program. Tbc-trans-ferrin is 21%, Tb-citrate is 62%, Tb-Asp is 15% when terbium concentration of Tb3+is smaller than 5×10-5mol/L.

Key words: Transferrin, Chelate reagent, Species, Terbium(Ⅲ)

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