高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (4): 539.

• 论文 • 上一篇    下一篇

手性化合物的毛细管胶束电动色谱分离研究

傅小芸, 吕建德, 徐秀珠, 王淼   

  1. 浙江大学应用化学研究所, 杭州 310027
  • 收稿日期:1998-08-03 出版日期:1999-04-24 发布日期:1999-04-24
  • 通讯作者: 傅小芸,女,38岁,博士,教授.
  • 作者简介:傅小芸,女,38岁,博士,教授.
  • 基金资助:

    国家自然科学基金(批准号:29675020);浙江省自然科学基金(296076)资助课题

Separation of Enantiomers by Micellae Electrokinetic Chromatography with Chiral Surfactant

FU Xiao-Yun, LU Jian-De, XU Xiu-Zhu, WANG Miao   

  1. Institute of Applied Chemistry, Zhejiang University, Hangzhou, 310027
  • Received:1998-08-03 Online:1999-04-24 Published:1999-04-24

摘要: 以4种不同的N-长链烷酰-L-氨基酸胶束为手性选择剂,对3种不同性质的手性化合物(α-氯代丙酰替苯胺,2-氨基-3-对硝基苯基-1,2-丙二醇和华法林)的毛细管胶束电动色谱分离进行研究.结果表明,手性表面活性剂中不同的氨基酸残基和烷基链的长度对分离影响较大;随手性表面活性剂浓度增加,溶质保留时间增大,分离度增加,不同溶质的最佳分离浓度在100~150mmol/L之间;pH对电中性手性化合物分离影响不大,但对酸性或碱性手性化合物的分离影响较大.在选定的条件下,3种样品均在20min内完全分离,分离柱效达1×105理论板数/m.

关键词: 电动色谱, 手性分离, N-长链烷酰-L-氨基酸, 胶束

Abstract: Several synthetic chiral surfactants such as Ndodecanoyl or NtetradecanoylLamino acids were used as the chiral selector respectively. The neutral, acidic and basic enantiomers such as chloropropionanilide, 2-amino-3-(4-nitrophenyl)-1,2-propanediol and warfarin were separated by MEKC. The effects of the type and concentration of chiral surfactants, pHof buffer solution on the enantiomeric separation were investigated. The results showed that the side group of amino acid and the chain length of hydrophobic alkyl group in chiral surfactant molecule had a significant influence on the separation. The concentration of chiral surfactant was an important factor influencing the enantiomeric separation. Both retention time and resolution increased with increasing the concentration of chiral surfactant. The optimum concentration was in the range from 100 to 150 mmol/Lfor the test samples. The pH of buffer solution had a dramatic effect on separation of the weak basic or acidic enantiomers, but little effect on neutral enantiomer. Under the selected condition, several test enantiomers were successfully separated. The separation efficiency was more than 1×105theoretical plates permeter.

Key words: Electrokinetic chromatography, Chiral separation, Long chain NacylL-amino acid, Micelles

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