高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (11): 1688.

• 论文 • 上一篇    下一篇

流动注射在线固相萃取预富集毛细管区带电泳测定血浆中的伪麻黄碱

陈恒武1, 方肇伦2   

  1. 1. 浙江大学西溪校区化学系, 杭州 310028;
    2. 东北大学分析科学研究中心, 沈阳 110006
  • 收稿日期:1999-03-05 出版日期:1999-11-24 发布日期:1999-11-24
  • 通讯作者: 陈恒武,男,51岁,博士,副教授.
  • 作者简介:陈恒武,男,51岁,博士,副教授.
  • 基金资助:

    浙江省自然科学基金(批准号:297060)资助课题

Determination of Pseudoephedrine in Human Plasma Using Capillary Zone Electrophoresis Coupled by Flow Injection on-line Solid Phase Extraction

CHEN Heng-Wu1, FANG Zhao-Lun2   

  1. 1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China;
    2. Analytical Science Research Centre, Northeastern University, Shenyang 110006, China
  • Received:1999-03-05 Online:1999-11-24 Published:1999-11-24

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被引次数

22

摘要： 研究了流动注射在线固相萃取与毛细管区带电泳联用系统(FI-SPE-CZE)应用于测定血浆中痕量盐酸伪麻黄碱的方法。通过脱线和在线试样净化、优化CZE分离条件等措施消除了血浆中共存组份的干扰;通过增加固相萃取的进样量、加大场放大电动进样量和柱上堆积的力度等,提高了联用系统的灵敏度。血浆试样沉淀蛋白后可连续进入FI-SPE-CZE系统进行自动的浓缩、分离与分析,检测限为12ng/mL,分析速度达10次/h.应用本方法成功地测定了志愿者口服正常剂量盐酸伪麻黄碱后的血药浓度,并试用于药物动力学的研究。

关键词: 毛细管电泳, 流动注射, 固相萃取, 伪麻黄碱, 血浆

Abstract: The previously developed hyphenated system of flow injection on-line solid phase extraction-capillary zone electrophoresis(FI-SPE-CZE) was adapted to the determination of trace pseudoephedrine in human plasma. Interference from the coexisting species was eliminated by off-line and on line sample cleaning-up, and by optimization of CZE separation conditions. The sensitivity of hyphenated system was improved by increasing of loaded sample volume and strengthening of field amplified stacking effect. Thus, plasma samples, after deproteinized by off-line precipitation, could be consecutively loaded onto the FI-SPE-CZE system, and trace amounts of pseudoephedrine be automatically preconcentrated, separated and determined by the system. The adapted FI-SPE-CZE system was successfully applied to determination of trace pseudoephedrine in volunteer s plasma samples for pharmacokinetic studies after administration of the drug with normal dosage.

Key words: Capillary electrophoresis, Flow injection, Solid phase extraction, Pseudoephedrine, Plasma

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