高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (8): 1232.

• 论文 • 上一篇    下一篇

柱前衍生非水毛细管电泳分离钴、镍和铜

刘笔锋, 刘良斌, 程介克   

  1. 武汉大学化学学院, 武汉, 430072
  • 收稿日期:1997-10-06 出版日期:1998-08-24 发布日期:1998-08-24
  • 通讯作者: 程介克
  • 作者简介:刘笔锋,男,25岁,博士研究生.

Separation of Cobalt, Nickel and Copper by Pre-column Derivatization Capillary Electrophoresis in Nonaqueous Medium

LIU Bi-Feng, LIU Liang-Bin, CHENG Jie-Ke   

  1. School of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1997-10-06 Online:1998-08-24 Published:1998-08-24

PDF (PC)

被引次数

16

摘要： 提出柱前衍生非水毛细管电泳分离金属离子的方法.应用新合成的2-(6-甲基-2-苯并噻唑偶氮)-5-二乙胺基酚作为柱前衍生试剂,乙醇和N,N-二甲基甲酰胺为非水溶剂,在6min内实现了钴、镍和铜的分离,灵敏度高,检测限分别为9.18×10-8mol/L、2.79×10-7mol/L和4.47×10-7mol/L.考察了柱前衍生条件、非水溶剂配比以及分离参数对分离的影响.

关键词: 柱前衍生, 非水毛细管电泳, 金属离子

Abstract: Separation of cobalt, nickel and copper elements as their 2-(6-methyl-2-benzothiazolyazo)-5-dimethylaminophenol complexes by capillary electrophoresis in nonaqueous medium was first presented. Ethanol and N,N-dimethylformamide(DMF) were evaluated for the effect of nonaqueous solvents on electroosmotic flow. The influence of the pre column derivitization conditions, some parameters such as choice and selection of organic solvents, concentration ratio in mixed nonaqueous medium, applied voltage on the separation were also investigated. Under the optimum conditions(buffer: 40 mmol/L, pH5.0 NaAc-HAc in ethanol(50%) and DMF(50%) mixture, 30 kV, 575 nm as detection wavelength), the three ions were separated in6min successfully, and detection limits of 9.18×10-8mol/L, 2.79×10-7mol/L and 4.47×10-7mol/Lfor cobalt, nickel and copper were achieved, respectively. The theoretical plate number reached up to 3.2×105/m.

Key words: Pre-column derivitization, Nonaqueous capillary electrophoresis, Metal ions

中图分类号: 

TrendMD: