高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (7): 1148.

• 研究快报 • 上一篇    下一篇

DNA分子结构的多态性研究(Ⅰ)--阳离子型表面活性剂诱导λ-DNA由线圈型向球型的转变

冯喜增, 林璋, 王琛, 白春礼   

  1. 中国科学院化学研究所, 北京, 100080
  • 收稿日期:1998-03-10 出版日期:1998-07-24 发布日期:1998-07-24
  • 通讯作者: 白春礼
  • 作者简介:冯喜增,男,36岁,博士后.
  • 基金资助:

    中国科学院"九五"重大科研基金(KJ-951-A1-502)和中国博士后科学基金资助课题.

The Investigation of the Various Structures of DNA Molecules(Ⅰ) The Coil Globe Transition of λ-DNA Induced by the Cation Surfactant

FENG Xi-Zeng, LIN Zhang, WANG Chen, BAI Chun-Li   

  1. Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080
  • Received:1998-03-10 Online:1998-07-24 Published:1998-07-24

摘要: 活性细胞众多的遗传信息均通过DNA链的碱基序列按一定的自身调节方式表达出各种蛋白质.DNA与各种不同性质的组分,如蛋白质、类脂及无机离子等的相互识别作用是确保细胞处于生理活性状态的重要调节机制。

关键词: 阳离子型表面活性剂, 扫描电子显微镜, 原子力显微镜

Abstract: The interaction between cetyltrimethylammonium bromide(CTAB) and λ-DNA was studied by SEM and AFM. It is clear that λ-DNA molecules exhibit discrete conformational change between the coil and globule states with the addition of cationic surfactant. When the concentration of CTAB is less than 2×10-6mol/L, all DNAmolecules exhibit the extended coil state. Whereas, when the CTAB concentration is higher than 8.0×10-6mol/L, only compacted DNA molecules in the globular state are observed. To study of coil globule transition, the process of the structural change from the coilinto the globule state is observed under the spatial gradient of the CTABconcentration. The formation of aggregates from two or more globules is noticed at high concentrations of surfactant above 8.0×10-5mol/L. The surfactant λ-DNA complex has a hydrophobic region akin to ordinary micelle.

Key words: Cationic surfactant, Scanning electron microscopy, Atomic force microscopy

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