高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (3): 391.

• 论文 • 上一篇    下一篇

双水相萃取法从发酵液中分离提取α-淀粉酶和蛋白酶的研究

焦庆才1, 刘茜1, 陈耀祖2   

  1. 1. 南京大学生物科学与技术系, 南京, 210093;
    2. 兰州大学化学系, 兰州, 730000
  • 收稿日期:1997-01-18 出版日期:1998-03-24 发布日期:1998-03-24
  • 通讯作者: 焦庆才,男,37岁,副教授.
  • 作者简介:焦庆才,男,37岁,副教授.
  • 基金资助:

    江苏省青年科技基金(批准号:Q94005)资助课题.

Studies on the Separation and Extraction of α-Amylase and Proteinase from Fermented Liquor of α-Amylase by Aqueous Two-phase Partitioning

JIAO Qing-Cai1, LIU Qian1, CHEN Yao-Zu2   

  1. 1. Department of Biological Science and Technology, Nanjing University, Nanjing, 210093;
    2. Department of Chemistry, Lanzhou University, Lanzhou, 730000
  • Received:1997-01-18 Online:1998-03-24 Published:1998-03-24

摘要: 以PEG/硫酸铵双水相体系,经一次萃取从α-淀粉酶发酵液中分离提取α-淀粉酶和蛋白酶.萃取最适宜的条件为15% PEG1000、20%(NH4)2SO4,pH=8,α-淀粉酶收率90%,分配系数为19.6;与蛋白酶的分离系数高达15.1,比活率为原发酵液的1.5倍,蛋白酶在水相中的收率高于60%.本文对反萃取条件进行了初步研究.与传统的提取法相比,本法可直接处理发酵粗滤液,提高工效,降低能耗和酶活损失.

关键词: &alpha, -淀粉酶, 蛋白酶, 聚乙二醇, 双水相体系, 萃取, 反萃取

Abstract: Aqueous two-phase system composed of PEG/(NH4)2SO4 was once to isolate α-amylase and proteinase from fermented liquor of α-amylase in this paper. The optimum extraction conditions were: 15% PEG1000, 20% (NH4)2SO4, pH=8, the partition coefficient of α-amylase was as high as 19.6 with a yield of 90%. The separation coefficients between α-amylase and proteinase were 15.1 and 1.5 folds increase of specific activity compared with those the original fermented liquor. The yield of proteinase in water phase was more than 60%. The preliminary study on back-extraction condition was done in this paper.

Key words: α-Amylase, Proteinase, Aqueous two-phase system, Polyethylene glycol, Extraction, Back-extraction

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