高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (10): 1584.

• 论文 • 上一篇    下一篇

固定化血红蛋白氧载体的研究(Ⅱ)——氧化纤维素共价键联血红蛋白

孔德领1, 贾永会1, 俞耀庭1, 童明容1, 王子晖2   

  1. 1. 南开大学分子生物学研究所, 生物活性材料开放研究实验室, 天津, 300071;
    2. 香港科技大学生物化学系, 香港九龙
  • 收稿日期:1997-11-04 出版日期:1998-10-24 发布日期:1998-10-24
  • 通讯作者: 孔德领,男,31岁,博士,副研究员.
  • 作者简介:孔德领,男,31岁,博士,副研究员.

Oxygen Carrier Based on Immobilized Hemoglobin(Ⅱ)——Hemoglobin Covalently Bond to Periodate Oxycellulose

KONG De-Ling1, JIA Yong-Hui1, YU Yao-Ting1, TONG Ming-Rong1, Wang Zi-Hui2   

  1. 1. Institute for Molecular Biology, Bioactive Materials Research Laboratory, Nankai University, Tianjin, 300071;
    2. Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong
  • Received:1997-11-04 Online:1998-10-24 Published:1998-10-24

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摘要： 以纤维素为载体,经高碘酸钠氧化后共价键联血红蛋白,制备氧载体.研究了氧化条件对血红蛋白固定量的影响.纤维素只有经过预氧化、碱处理和再氧化三步活化反应,才能使血红蛋白大量固定化,每克纤维素固定血红蛋白量达1.0g.氧载体稳定性好,血红蛋白不脱落.经铁氰化钾氧化,氧载体放氧效率为33.1%.

关键词: 氧载体, 氧化纤维素, 血红蛋白固定化, 人工鳃

Abstract: Cellulose can be easily oxidized to oxycellulose with periodic acid, which can serve as a carrier for the immobilization of hemoglobin(Hb) acting like a bionic gill, to extract oxygen from sea water, which may provide a source of oxygen for human life support in an undersea environment. The objective of the present paper is to study the possibility of using modified cellulose derivatives as a carrier to immobilize a high amount of Hb.

Key words: Oxygen carrier, Oxycellulose, Immobilized hemoglobin, Artificial gill

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