高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (6): 920.

• 研究简报 • 上一篇    下一篇

使用高效疏水作用色谱和凝胶色谱纯化重组人干扰素-γ

郭立安1, 刘树林1, 朱宝泉2   

  1. 1. 第四军医大学中心实验室, 西安, 710032;
    2. 上海医药工业研究院, 上海, 200040
  • 收稿日期:1996-07-29 出版日期:1997-06-24 发布日期:1997-06-24
  • 通讯作者: 郭立安, 男, 35岁, 上海医药工业研究院博士研究生, 讲师.
  • 作者简介:郭立安, 男, 35岁, 上海医药工业研究院博士研究生, 讲师.
  • 基金资助:

    中国人民解放军85青年基金

Purification of Recombinant Human Interferon-γ by High-Performance Hydrophobic Interaction Chromatography and Size Exclusion Chromatography

GUO Li-An1, LIU Shu-Lin1, ZHU Bao-Quan2   

  1. 1. The Centre Labaratory, 4th Mlitary Medical University, Xi'an, 710032;
    2. Shanghai Institute of Pharmaceutical Industry, Shanghai, 200040
  • Received:1996-07-29 Online:1997-06-24 Published:1997-06-24

关键词: 高效疏水作用色谱, 重组人干扰素-&gamma, 纯化, 凝胶色谱

Abstract: Adownstream purification procedure for recombinant human interferon-γ(rhIFN-γ) expressed in E. coli was described. An essentially two-step chromatographic purification procedure, i. e., size exclusion chromatography(SEC) and high-performance hydrophobic in-teraction chromatography (HPHIC), was used for purification of homogeneity of rhIFN-γ from the inclusion body. The specific activity of purified rhIFN-Ywas 1.0×108 IU/mg pro-tein. The product of purification rhIFN-γ was analyzed by an analytical high-per formance SECand a significant sigle symmetrical peak had been found. The purity of purified rhIFN-Ywas greater than 95% from analysis, determination by analytical HPSECand SDS-PAGEwith Coomssie Blue. The molecular weight was ca. 15000. It was shown that this procedure was an effective method for purifying rhIFN-γ.

Key words: High-performance hydrophobic interaction chromatography, Recombinant human interferon-γ, Purification, Size exclusion chromatography

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