高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (5): 753.

• 论文 • 上一篇    下一篇

线粒体和亚线粒体体外代谢及漆酶影响的微量热研究

熊亚1, 谭智群2, 刘义1, 潘正君2, 吴鼎泉1, 康立山3, 屈松生1   

  1. 1. 武汉大学化学系, 武汉, 430072;
    2. 武汉大学环境科学系, 武汉, 430072;
    3. 武汉大学软件工程国家重点实验室, 武汉, 430072
  • 收稿日期:1996-05-23 出版日期:1997-05-24 发布日期:1997-05-24
  • 通讯作者: 熊亚, 男, 35岁, 博士研究生.
  • 作者简介:熊亚, 男, 35岁, 博士研究生.
  • 基金资助:

    国家自然科学基金

Studies on the Metabolism of Mitochondria and Sub-mitochondria and the Effect of Laccase on It by Microcalorimetry

XIONG Ya1, TAN Zhi-Qun2, LIU Yi1, PAN Zheng-Jun2, WU Ding-Quan1, KANG Li-Shan3, QU Song-Sheng1   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. Department of Environmental Science, Wuhan University, Wuhan, 430072;
    3. Key State Laboratory of Soft Engineering, Wuhan University, Wuhan, 430072
  • Received:1996-05-23 Online:1997-05-24 Published:1997-05-24

摘要: 首次将微量热用于亚细胞器层次的研究,测定了鲤鱼肝脏线粒体和亚线粒体体外代谢的热谱及其漆树漆酶的影响,并用MTD方程和演化计算技术对它们的代谢热动力学参数进行了全局优化.

关键词: 线粒体, 漆树漆酶, 微量热, 演化计算

Abstract: Microcalorimetry was first applied to investigating sub-cell organ.The metabolism of mitochondria and sub-mitochondria, and the effect of laccase on sub-mito-chondria were studied by microcalorimetry.Their metabolism thermokinetic parameters were obtained by the MTDequation and evolutionary optimization.It is found that the metabolism rate constant and the heat of mitochondria are almost the same as that of sub-mitochondria, and laccase may enormously speed the metabolism of mitochondria although the metabolism heat of sub-mitochondria-laccase system is almost the same as sub-mitochondria.

Key words: Mitochondria, Laccase, Microcalorimetry, Evolutionary computation

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