高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (08): 1771.doi: 10.3969/j.issn.0251-0790.2012.08.025

• 物理化学 • 上一篇    下一篇

采用微量热法研究重金属离子对脲酶催化水解反应的影响

王丽萍1, 胥义1, 郑艺华2, 李代禧1, 徐斐1   

  1. 1. 上海理工大学生物系统热科学研究所, 上海 200093;
    2. 青岛大学机电工程学院, 生物系统热科学研究所, 青岛 266071
  • 收稿日期:2011-12-12 出版日期:2012-08-10 发布日期:2012-08-10
  • 通讯作者: 胥 义, 男, 博士, 副教授, 主要从事生物系统热科学技术研究. E-mail: xu_hongyi@263.net E-mail:xu_hongyi@263.net
  • 基金资助:

    国家自然科学基金(批准号: 50906056, 50806036); 上海市教委创新项目(批准号: 11YZ111)和上海市科委启明星项目(批准号: 08QA14052)资助.

Urease-catalyzed Reactions Inhibited by Heavy Metals Ion with Micro-calorimetry

WANG Li-Ping1, XU Yi1, ZHENG Yi-Hua2, LI Dai-Xi1, XU Fei1   

  1. 1. Institute of Biothermal Science & Technology, University of Shanghai for Science & Technology, Shanghai 200093, China;
    2. Institute of Biothermal Science & Technology, College of Electromechanical Engineering, Qingdao University, Qingdao 266071, China
  • Received:2011-12-12 Online:2012-08-10 Published:2012-08-10

摘要: 利用微量热计Micro DSCⅢ对重金属离子影响脲酶催化水解反应的过程进行研究, 并探讨重金属离子联合抑制作用对脲酶催化反应的影响. 结果表明, 在25℃下, 重金属离子对脲酶水解反应热有影响, 在广泛浓度范围(0.1, 1, 10, 100和103 mg/L)内, 这种影响不仅表现在抑制作用方面, 而且会促进其水解反应的进行, 如铜离子在100和1000 mg/L时抑制率分别为(0.49±0.20)%和(-11.93±1.34)%. 重金属离子浓度与抑制率具有显著或极显著的相关性, 抑制效果的顺序为砷离子>铅离子>镉离子>铜离子. 当重金属离子联合抑制时, 其抑制效果与抑制率较高的重金属离子的抑制作用接近.

关键词: 微量热法, 酶抑制法, 重金属离子, 抑制率, 联合抑制作用

Abstract: Inhibiting effects of heavy metal ions on urease-catalyzed reaction were investigated with micro-calorimetry. Further, the joint inhibition effects of two heavy metal ions on urease-catalyzed reaction were also conducted. The results indicate that, with a wide range of concentration(0.1, 1, 10, 100 and 103 mg/L), heavy metal ions not only inhibit urease hydrolysis reaction, but also can promote this reaction in some concentration range. For instance, inhibition rate is (0.49?0.20)% and (-11.93?1.34)% when Cu2+concentration is 100 and 1000 mg/L, respectively. The correlation between urease inhibition rate and concentration of heavy metal ion is very significant, and the inhibition effects are: As3+>Pb2+>Cd2+>Cu2+. The joint inhibition is close to that with higher inhibition when two heavy metal ions are used together. This work proves that the micro-calorimetry may be a potential method to rapidly and accurately detect heavy metals.

Key words: Micro-calorimetry, Enzyme inhibition, Heavy metal ion, Inhibition rate, Joint inhibition

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