高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (1): 52.

• 研究简报 • 上一篇    下一篇

葡萄糖氧化酶催化荧光测定的非酶体系研究

李庆阁, 许金钩, 黄贤智, 陈国珍   

  1. 厦门大学肿瘤细胞工程国家专业实验室, 厦门大学化学系, 厦门, 361005
  • 收稿日期:1996-02-02 出版日期:1997-01-24 发布日期:1997-01-24
  • 通讯作者: 李庆阁. 男. 29岁, 博士, 讲师.
  • 作者简介:李庆阁. 男. 29岁, 博士, 讲师.

Non-enzymatic System for Catalytic Fluorometric Determination of Glucose Oxidase

LI Qing-Ge, XU Jin-Gou, HUANG Xian-Zhi, CHEN Guo-Zhen   

  1. The State Laboratory for Tumor Cell Engineering, Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1996-02-02 Online:1997-01-24 Published:1997-01-24

关键词: 葡萄糖氧化酶, 荧光测定, 羟基自由基

Abstract: Anovel non-enzymatic method for the determination of oxidases and their sub-strates was proposed.The principle was examined by fluorometric determination of glucose oxidase(GOD).Using EGTA (NH4)2Fe (SO4), as modified Fenton reagent, H2O2, the prod-uct of GODcatalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GODwas thus measured according to the reaction rates.The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-

Key words: Glucose oxidase, Fluorometry determination, Hydroxyl radical

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