高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (11): 1693.

• 论文 • 上一篇    下一篇

HPLC分离化学发光检测药物中的马钱子碱、士的宁和麻黄类生物碱

朱龙, 封满良, 万秀琴, 吕九如   

  1. 陕西师范大学化学系, 西安, 710062
  • 收稿日期:1995-11-09 出版日期:1996-11-24 发布日期:1996-11-24
  • 通讯作者: 吕九如
  • 作者简介:朱龙,男,24岁.硕士研究生.
  • 基金资助:

    国家自然科学基金

Chemiluminescence Determination of Brucine,Strychnine and Ephedrines Based on HPLC Separation

ZHU Long, FENG Man-Liang, WAN Xiu-Qin, LU Jiu-Ru   

  1. Department of Chemistry, Shaanxi Normal University, Xian, 710062
  • Received:1995-11-09 Online:1996-11-24 Published:1996-11-24

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摘要： 在KMnO4-H+-还原剂体系中研究了马钱子碱、士的宁和麻黄类生物碱的化学发光(CL)行为,建立了其快速灵敏的流动注射化学发光分析方法,将此法与HPLC联用,实现了上述物质的选择性测定,该方法的检出限为1.0×10-10g/mL马钱子碱、1.0×10-8g/mL士的宁、1.0×10-5g/mL麻黄碱和伪麻黄碱,测定的相对标准偏差小于3%,HPLC流动相的脉动及其中的溶解氧不影响测定的精密度。此法已用于一些药物中马钱子碱等生物碱的测定,回收率为94%～101%.

关键词: HPLC, 化学发光分析, 高锰酸钾, 生物碱

Abstract: In this paper,the chemiluminescence reaction of brucine,strychnine and ephedrines in KMnO4-H+-reductant system has been characterized with respect to their ability to act as a rapid and sensitive chemiluminescence determination system of the above alkaloids.This method has been connected with HPLC which fitted with a C18 column for separation so as to determine these alkaloids in sample.The detection limits are 1.0×10-10g/mLof brucine,1.0×10-8g/mL of strychnine and 1.0×10-5 g/mLof ephedrines, respectively.The relative standard deviation is less than 3% and recovery is 94%～101%.Since the dissolved oxygen did not affect the determination,this method provide advantages of wider linear range,high sensitivity and easy to operate.

Key words: HPLC, Chemiluminescence, potassium permanganate, Alkaloids

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