高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (9): 1335.

• 论文 • 上一篇    下一篇

猪胰脂肪酶的固定化及其在有机相中催化丁酸甲酯和正丁醇的酯交换反应研究

谢志东1, 何炳林1, 吕宪禹2   

  1. 1. 南开大学高分子化学研究所, 天津, 300071;
    2. 南开大学生物系
  • 收稿日期:1993-11-22 修回日期:1994-03-28 出版日期:1994-09-24 发布日期:1994-09-24
  • 通讯作者: 谢志东,男,27岁,博士,助理研究员.
  • 作者简介:谢志东,男,27岁,博士,助理研究员.
  • 基金资助:

    国家自然科学基金

Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and1-Butanol in Organic Systems

XIE Zhi-Dong1, HE Bing-Lin1, LU Xian-Yu2   

  1. 1. Institute of Polymer Chemistry, Nankai University, Tianjin, 300071;
    2. Department of Biology, Nankai University
  • Received:1993-11-22 Revised:1994-03-28 Online:1994-09-24 Published:1994-09-24

摘要: 考察了大孔苯乙烯-二乙烯苯交联共聚物的交联度、致孔剂量及胺化试剂对载体固定化猪胰脂肪酶的影响。选择出一种最佳载体对猪胰脂肪酶进行固定化,对比了自由酶和固定化酶在有机相中催化丁酸甲酯和正丁醇的酯交换反应。结果表明,酶经固定化后催化反应活力比自由酶提高近1倍。

关键词: 聚苯乙烯, 猪胰脂肪酶, 固定化, 有机相, 酯交换反应

Abstract: Series of macroporous cross-linked polymers of styrene with divinylbenzene and their functionalization products were synthesized. Effects of degree of cross-linking, amount of pore-generating agent and different aminating agents on immobilization of porcine pancreas lipase(PPL)were studied.The best carrier was selected.Transesterification of methyl butyrate with 1-butanol was catalyzed by the free and immobilized PPL, it was found that the catalytic activity of immobilized PPLin organic systems was two times as high as that of the free PPL.

Key words: Polystyrene, Porcine pancreas lipase, Immobilization, Organic systems, Transesterification

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