高等学校化学学报

高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (12): 1757.

• 论文 • 上一篇    下一篇

以四硫富瓦烯为电子媒介体的电流式葡萄糖传感器

刘海鹰, 雷呈宏, 邓家祺   

  1. 复旦大学化学系, 上海, 200433
  • 收稿日期:1993-12-24 修回日期:1994-04-20 出版日期:1994-12-24 发布日期:1994-12-24
  • 通讯作者: 邓家棋.
  • 作者简介:刘海鹰,男,28岁,博士研究生.
  • 基金资助:

    国家自然科学基金;中国科学院长春应用化学研究所电分析化学开放实验室资助

Amperometric Mediated Biosensor Based on Tetrathiafulvalene as Electron Shuttle Between Glucose Oxidase and Glassy Carbon

LIU Hai-Ying, LEI Cheng-Hong, DENG Jia-Qi   

  1. Department of Chemistry, Fudan University, Shanghai, 200433
  • Received:1993-12-24 Revised:1994-04-20 Online:1994-12-24 Published:1994-12-24

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被引次数

1

摘要： 用四硫富瓦烯(TTF)作为酶与电极之间的电子传递体,通过Nafion水溶液把葡萄糖氧化酶固定在Nafion-TTF修饰玻碳电极上,最后在电极上修饰一层Nafion膜,制备成葡萄糖传感器。Nafion膜不仅能防止四硫富瓦烯流失,而且能把抗坏血酸、尿酸等电活性物质阻挡在电极外,防止其干扰,同时具有防污性能。通过实验表明TTF+1、TTT+2都能够氧化葡萄糖氧化酶中的辅酶(FADH2).该传感器的线性范围为3.0×10-4～1.0×10-2mol/L,响应时间小于20s.

关键词: 生物传感器, Nafion水溶液, 四硫富瓦烯, 电子媒介体, 葡萄糖氧化酶

Abstract: Amperometric mediated biosensor for glucose has been developed by using aqueous Nafion to immobilize glucose oxidase on Nafion-TTFmodlfied glassy carbon. It is further coated by Nafion, a perfluorosulphonated ion-exchange polymer. Nafion coating is not detrimental to the enzyme but to forestall electrochemically active anionic interferences such as ascorbate and urate from reaching the electrode.The polymer coating also avoids tetrathiafulvalene's leaking and keeps the biosensor from fouling agents. For the first time it has been experimentally proved that both TTF+ and TTF2+ can oxidise FADH2 of glucose oxidase. The biosensor responds rapidly to glucose in less than20 seconds and it's calibration curve is linear from 3.0×10-4 to 1.0×10-2 mol/L.

Key words: Biosensor, Aqueous Nafion, Tetrathiafulvalene, Mediator, Glucose oxidase

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