高等学校化学学报

高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (7): 914.

• 研究论文 • 上一篇    下一篇

高压液相色谱磷钼杂多酸修饰电极安培检测儿茶酚胺类神经递质的研究

金利通, 刘彤, 周满水, 方禹之   

  1. 华东师范大学化学系, 上海 200062
  • 收稿日期:1992-08-22 修回日期:1993-01-14 出版日期:1993-07-24 发布日期:1993-07-24
  • 通讯作者: 金利通.
  • 作者简介:第一作者:男, 56, 教授.
  • 基金资助:

    国家自然科学基金

Determination of Catecholamines by Catalytic Oxidation at 1:12 Molybdophosphate CME in Liquid Chromatography

JIN Li-Tong, LIU Tong, ZHOU Man-Shui, FANG Yu-Zhi   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1992-08-22 Revised:1993-01-14 Online:1993-07-24 Published:1993-07-24

PDF (PC)

被引次数

31

摘要： 研究了磷钼杂多酸修饰电极对去甲肾上腺素(NE)、肾上腺素(E)和多巴胺(DA)等儿茶酚胺类神经递质的催化氧化作用,探讨了催化机理,并采用高压液相色谱电化学方法对其进行了分离检测,NE、DA和E的线性范围(mol)依次为8.0×10-11～2.0×10-8、8.0×10-11～2.0×10-8、4.0×10-11～2.0×10-8;检测限(mol))依次为4.0×10-11、4.0×10-11、2.0×10-11.7次平行测定的相对标准偏差(％)依次为1.6、2.0和4.5.将此法用于鼠脑组织中神经递质的测定,获得满意的结果.

关键词: 磷钼杂多酸修饰电极, 液相色谱电化学, 去甲肾上腺素, 肾上腺素, 多巴胺

Abstract: An electrode modified with 1:12 molybdophosphate (PMo12 CME) was prepared.Its high electrocatalytic effect on the oxidation of catecholamines (noradrenaline(NE), adrenaline (E) and dopamine(DA)) was described, and the mechanism was discussed.LCECwith PMo12 CMEwas used to separate and detect catecholamines.The linear range of detection is 8.0×10-11-2.0×10-8 mol for NEand DA, 4.0×10-11-2.0×10-8 mol for E.The detection limit is 4.0×10-11 mol for NEand DA, 2.0×10-11 mol for E.The relative standard deviation for seven parallel determination is 1.6% for NE, 2.1% for DAand 4.5% for E.The noradrenaline in rat brain was detected and the results are satisfactory.

Key words: 1:12 Molybdophosphate modified electrode, Liquid chromatography electrochemistry, Noradrenaline, Adrenaline, Dopamine

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